Evidence map›Paper›PMID 42783179›Full record

ArticleBiosensors2026

Combining Asymmetric PCR with PAM-Independent Cas12a Analysis of Single-Stranded Amplicons for On-Site Nucleic Acid Testing.

Konstantin G Ptitsyn, Olga S Timoshenko, Svetlana A Khmeleva, Leonid K Kurbatov, Adelina A Stepanova, Evgenia M Nikiforova, Elena V Suprun, Sergey P Radko, Andrey V Lisitsa

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biosensors, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Konstantin G PtitsynV.N. Orekhovich Institute of Biomedical Chemistry, 10 Pogodinskaya St., 119121 Moscow, Russia.ORCID 0000-0002-3197-6415
Olga S TimoshenkoV.N. Orekhovich Institute of Biomedical Chemistry, 10 Pogodinskaya St., 119121 Moscow, Russia.
Svetlana A KhmelevaV.N. Orekhovich Institute of Biomedical Chemistry, 10 Pogodinskaya St., 119121 Moscow, Russia.ORCID 0000-0001-6224-4041
Leonid K KurbatovV.N. Orekhovich Institute of Biomedical Chemistry, 10 Pogodinskaya St., 119121 Moscow, Russia.
Adelina A StepanovaV.N. Orekhovich Institute of Biomedical Chemistry, 10 Pogodinskaya St., 119121 Moscow, Russia.
Evgenia M NikiforovaV.N. Orekhovich Institute of Biomedical Chemistry, 10 Pogodinskaya St., 119121 Moscow, Russia.
Elena V SuprunThe Chemistry Faculty, M.V. Lomonosov Moscow State University, 1/3 Lenin Hills, 119991 Moscow, Russia.ORCID 0000-0002-2863-8562
Sergey P RadkoV.N. Orekhovich Institute of Biomedical Chemistry, 10 Pogodinskaya St., 119121 Moscow, Russia.ORCID 0000-0002-0519-1745
Andrey V LisitsaV.N. Orekhovich Institute of Biomedical Chemistry, 10 Pogodinskaya St., 119121 Moscow, Russia.

Funding

Ministry of Education and Science of Russian Federation Program for Basic Research in the Russian Federation for a long-term period (2021-2030) (No. 122030100170-5)
6 · The paper itself

Abstract

A biosensing system relying on asymmetric PCR (aPCR) and PAM-independent recognition of single-stranded DNA amplicons by a Cas12a/gRNA complex is adapted for on-site nucleic acid testing (NAT). aPCR was performed on a low-cost, portable, smartphone-controlled thermocycler operating in endpoint mode. Subsequently, a selective visual readout of the aPCR outcome was achieved using a Cas12a-based assay. A mechanism to prevent false positives was incorporated into the aPCR/Cas12a biosensing system by introducing uracil into the amplicons produced during amplification.

Indexed as

Biosensing TechniquesCRISPR-Associated ProteinsPolymerase Chain ReactionBacterial ProteinsDNA, Single-StrandedEndodeoxyribonucleasesPectobacteriumBacterial ProteinsCas12a proteinCRISPR-Associated ProteinsDNA, Single-StrandedEndodeoxyribonucleasesasymmetric PCRCas12a nucleaseon-site nucleic acid testingP. polaris detectionprotospacer adjacent motifsingle-stranded DNAuracil-containing ampliconsvisual readout

Identifiers

PMID42783179
PMCPMC13604837

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.