ArticleJournal of bioenergetics and biomembranes2026
M2-Exo-derived miR-1227-5p protects against periodontitis through inhibiting USP2-dependent deubiquitination of Drp1.
Article in Journal of bioenergetics and biomembranes, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Periodontitis is a common chronic inflammatory disease with an exaggerated host immune response. M2 type macrophages exert protective properties in periodontitis. This study aimed to investigate the role of M2-Exo-derived miR-1227-5p in periodontitis. M2-Exo were verified using TEM and NTR assay. miRNA and mRNA levels were determined using RT-qPCR. periodontal ligament stem cells (PDLCs) were exposed to LPS in establish in vitro periodontitis model. Gene expression was determined using Western blot and immunofluorescence. The binding sites of miR-1227-5p on USP2 were predicted by TargetScan and verified by luciferase, RNA pull-down, and RIP assays. Deubiquitination of Drp1 was determined using in vitro ubiquitination assay. Cellular behaviors of PDLCs were analyzed by CCK-8, alizarin red staining, and ALP staining. LPS exposure induced mitochondrial dysfunction and suppressed the osteogenesis of PDLCs. M2-Exo-derived miR-1227-5p improved mitochondrial function and promoted the proliferation and osteogenesis of PDLCs. miR-1227-5p directly targeted USP2. USP2 overexpression reversed the effects of miR-1227-5p and improved mitochondrial function and the osteogenesis of PDLCs. Moreover, miR-1227-5p targeted USP2 to inhibit the deubiquitination of Drp1, improving mitochondrial function. Furthermore, M2-Exo-derived miR-1227-5p alleviates periodontitis via regulating USP2/Drp1. Therefore, M2-Exo hold great potential for future clinical applications.
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