Evidence map›Paper›PMID 42782444›Full record

ReviewAdvances in experimental medicine and biology2026

Synthetic Cell-Based Approaches to Dissect Autonomous Cellular Oscillators: Recent Applications on the Cell Cycle.

Yeonghoon Kim, Franco Tavella, Qiong Yang

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In one paragraph

Review in Advances in experimental medicine and biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Yeonghoon KimDepartment of Biophysics, University of Michigan, Ann Arbor, MI, USA.
Franco TavellaDepartment of Biophysics, University of Michigan, Ann Arbor, MI, USA.
Qiong YangDepartment of Biophysics, University of Michigan, Ann Arbor, MI, USA. qiongy@umich.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cell-free extracts have proven to be a tremendous tool for studying cell cycle regulation. This chapter reviews recent advances that expand upon this platform by incorporating synthetic cell-like compartments to encapsulate functional cell cycle regulators. These synthetic approaches allow for a programmable reconstitution of self-sustained autonomous oscillators, including that of mitosis, exhibiting a diverse range of biochemical and physical properties. The high throughput and manipulability of this system provide a comprehensive tool to systematically analyze the single-cell dynamics of this autonomous cell-cycle oscillator across broader yet finer-grained parametric, temporal, and spatial scales. We highlight key applications from the past few years and demonstrate how extending the well-established extract-based research with synthetic systems opens new avenues for tackling unexplored questions regarding cell cycle oscillations and their emergent phenomena.

Indexed as

Artificial CellsBiological ClocksCell CycleAnimalsCDC2 Protein KinaseCell-Free SystemHumansMitosisSingle-Cell AnalysisCDC2 Protein KinaseBistable switchCell-free extract systemsCyclin B-Cdk1 networkEnergy flowHigh-throughput single-cell perturbation and analysisMitotic wavesParameter space mappingPositive and negative feedback loopsPP2A-Greatwall pathwayRelaxation oscillatorRobustness and tunabilitySaddle-node bifurcationSpatiotemporal synchronizationTemperature scalingWater-in-oil microemulsions

Identifiers

PMID42782444

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.