Evidence map›Paper›PMID 42781324›Full record

ArticleChemical science2026

Electric field-dependent excited-state twisting in donor-acceptor thiazolothiazole dyes establishes a new class of cell membrane voltage sensors.

Pranamita Chakraborti, Rui Fu, Lucia M Wert, Charlie A L Darby, Joy D Amuzu, Daphne Oettinger, Aditya Nandy, Yamuna Krishnan, Michael G Walter

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chemical science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Pranamita ChakrabortiDepartment of Chemistry, University of North Carolina at Charlotte Charlotte NC 28223 USA Michael.Walter@charlotte.edu.ORCID https://orcid.org/0009-0006-3955-2760
Rui FuDepartment of Chemistry, The University of Chicago Chicago IL 60637 USA yamuna@uchicago.edu.
Lucia M WertDepartment of Chemistry, University of North Carolina at Charlotte Charlotte NC 28223 USA Michael.Walter@charlotte.edu.
Charlie A L DarbyDepartment of Chemistry, University of North Carolina at Charlotte Charlotte NC 28223 USA Michael.Walter@charlotte.edu.
Joy D AmuzuDepartment of Chemistry, University of North Carolina at Charlotte Charlotte NC 28223 USA Michael.Walter@charlotte.edu.
Daphne OettingerDepartment of Chemistry, The University of Chicago Chicago IL 60637 USA yamuna@uchicago.edu.
Aditya NandyDepartment of Chemical and Biomolecular Engineering, University of California, Los Angeles Los Angeles CA 90095 USA aditya.nandy@ucla.edu.ORCID https://orcid.org/0000-0001-7137-5449
Yamuna KrishnanDepartment of Chemistry, The University of Chicago Chicago IL 60637 USA yamuna@uchicago.edu.ORCID https://orcid.org/0000-0001-5282-8852
Michael G WalterDepartment of Chemistry, University of North Carolina at Charlotte Charlotte NC 28223 USA Michael.Walter@charlotte.edu.ORCID https://orcid.org/0000-0002-9724-265X

Funding

Intracellular Electrophysiology: An electrochemical atlas of organellesDP1GM149751 · NIGMS · UNIVERSITY OF CHICAGO · PI KRISHNAN, YAMUNA · 2022 to 2025
$5.7M
Calcium homeostasis in organellesR01NS112139 · NINDS · UNIVERSITY OF CHICAGO · PI KRISHNAN, YAMUNA · 2020 to 2024
$1.8M
Synthesis and Exploration of Highly Fluorescent Thiazolothiazole Molecular Sensors for Probing Membrane Potential DynamicsR15GM140392 · NIGMS · UNIVERSITY OF NORTH CAROLINA CHARLOTTE · PI WALTER, MICHAEL G. · 2020 to 2025
$1.0M
NIGMS NIH HHS DP1 GM149751NIGMS NIH HHS R15 GM140392NINDS NIH HHS R01 NS112139
6 · The paper itself

Abstract

Voltage sensitive dyes (VSDs) are versatile and powerful reporters of the membrane potential across cell membranes. Thiazolothiazole (TTz) dyes are a promising new class of VSDs with high photostability and low cytotoxicity. However, the mechanistic basis of their voltage sensitivity is unknown. To address this, we developed a new generation of asymmetrically substituted TTz dyes (asym-TTz). They exhibit strong solvatofluorochromism with large Stokes shifts and exceptionally large changes in dipole moments (23-29 D), representing an 80% increase over those of previously reported TTz VSDs. An asym-TTz derivative, TwistTz-P, bearing a carboxyphenyl acceptor group, shows sustained cellular membrane localization and a voltage sensitivity of ∼10% Δ

Identifiers

PMID42781324
PMCPMC13598645

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.