ArticleResearch square2026
Electroporation outcomes in human Jurkat cells are affected by exposure to simulated microgravity.
Article in Research square, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Authors and funding
5 authors.
Funding
Abstract
Electroporation (EP) is commonly used to permeabilize cell membranes to deliver nucleic acids and other biomolecules. EP-based methodologies have the potential to support in-space biomanufacturing; however, it is currently unclear whether cellular adaptations to microgravity influence EP outcomes. To investigate this, human T-lymphocyte (Jurkat) cells were exposed to simulated microgravity (SμG) in a rotating wall vessel (RWV) bioreactor prior to EP to deliver exogenous fluorescent molecules or plasmid DNA. We observed that two hours of SμG exposure led to a 20% decrease in fluorophore uptake and a 31% decrease in transfection efficiency compared to standard gravity controls for several voltages. To explore underlying mechanisms, the effects of SμG on actin organization were assessed using a phalloidin stain, which revealed increased cell area and cortical actin thickness following SμG. Actin targeting drugs jasplakinolide and latrunculin B were used to promote or destabilize F-actin, respectively, and modulated cell area and cortical actin thickness. Promoting F-actin also led to decreased transfection efficiency, mimicking the effect of SμG, while destabilizing F-actin during SμG showed a small increase in transfection efficiency, slightly reversing the impact of SμG. Our results demonstrate that adaptations to microgravity exposure can influence transfection efficiency via cytoskeletal reorganization.
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Registered trials
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