Evidence map›Paper›PMID 42779884›Full record

ArticlebioRxiv : the preprint server for biology2026

Longitudinal single-cell and spatial transcriptomics reveals intratumor heterogeneity, therapeutic response, and comparative value of canine marginal zone lymphoma.

G E Walker, M Macchietto, K Reid, L E Burt, A Winter, S Pracht, M Buettner, V Penza, C Yung, R Dicovitsky and 11 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors.

G E WalkerMasonic Cancer Center, University of Minnesota, Minneapolis, MN, USA.
M MacchiettoResearch Informatics, Minnesota Supercomputing Institute, University of Minnesota, Minneapolis, MN, USA.
K ReidClinical Investigation Center, College of Veterinary Medicine, University of Minnesota, St. Paul, MN, USA.
L E BurtMasonic Cancer Center, University of Minnesota, Minneapolis, MN, USA.
A WinterClinical Investigation Center, College of Veterinary Medicine, University of Minnesota, St. Paul, MN, USA.
S PrachtClinical Investigation Center, College of Veterinary Medicine, University of Minnesota, St. Paul, MN, USA.
M BuettnerClinical Investigation Center, College of Veterinary Medicine, University of Minnesota, St. Paul, MN, USA.
V PenzaDepartment of Molecular Medicine & Clinical Translation, Mayo Clinic, Rochester, MN, USA.
C YungDepartment of Veterinary Clinical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, MN, USA.
R DicovitskyDepartment of Veterinary Clinical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, MN, USA.
A KuzmikDepartment of Veterinary Clinical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, MN, USA.
D A ValleraMasonic Cancer Center, University of Minnesota, Minneapolis, MN, USA.
K Demos-DaviesDepartment of Veterinary Clinical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, MN, USA.
D M SeeligMasonic Cancer Center, University of Minnesota, Minneapolis, MN, USA.
A BorgattiMasonic Cancer Center, University of Minnesota, Minneapolis, MN, USA.
M HensonMasonic Cancer Center, University of Minnesota, Minneapolis, MN, USA.
C FeiockDepartment of Veterinary Clinical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, MN, USA.
J F ModianoMasonic Cancer Center, University of Minnesota, Minneapolis, MN, USA.
S NaikDepartment of Molecular Medicine & Clinical Translation, Mayo Clinic, Rochester, MN, USA.
A L SarverMasonic Cancer Center, University of Minnesota, Minneapolis, MN, USA.
A E TreefulMasonic Cancer Center, University of Minnesota, Minneapolis, MN, USA.

Funding

Women's CancerP30CA077598 · NCI · UNIVERSITY OF MINNESOTA TWIN CITIES · PI Timothy C. Hallstrom · 1998 to 2026
$100.4M
University of Minnesota Clinical and Translational Science Institute (UMN CTSI)UM1TR004405 · NCATS · UNIVERSITY OF MINNESOTA · PI Bruce R Blazar, Damien A Fair · 2023 to 2026
$30.8M
Translation of a novel combination therapy approach for non-Hodgkin lymphomaU01CA272257 · NCI · MAYO CLINIC ROCHESTER · PI JAIME F. MODIANO, Shruthi Naik · 2022 to 2026
$3.2M
NCATS NIH HHS UM1 TR004405NCI NIH HHS P30 CA077598NCI NIH HHS U01 CA272257
6 · The paper itself

Abstract

Diffuse B-cell lymphomas are the most prevalent canine hematologic malignancies, and their clinical presentation resembles that of human B-cell non-Hodgkin lymphomas (NHLs). However, a lack of a clear subtyping framework perpetuates imprecise treatment approaches that fail to address mechanisms of therapy resistance. Here, we employed deep phenotyping via serial sampling and longitudinal transcriptomics to evaluate intratumoral composition and therapy response in a 6-year-old neutered male goldendoodle with stage 5A marginal zone lymphoma (MZL). Following sequential treatment consisting of a single IV dose of oncolytic vesicular stomatitis virotherapy (VSV) and standard CHOP chemotherapy 30 days later, we performed scRNAseq on seven serial lymph node biopsies and spatial sequencing on a resection taken 10 days post-VSV treatment. B cells (>90%) comprised three transcriptionally distinct and temporally stable subpopulations, with specific copy number profiles and robust spatial organization despite disrupted lymph node architecture. Analysis across subpopulations revealed downregulation of the quiescence program including

Identifiers

PMID42779884
PMCPMC13596568

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.