Evidence map›Paper›PMID 42778925›Full record

ArticleMicrobial cell factories2026

Eliminating manganese sensitivity and boosting citric acid production in Aspergillus niger through transporters manipulation.

Alexandra Márton, Josee Vandal, Vivien Bíró, Erzsébet Fekete, Erzsébet Sándor, Béla Kovács, Adrian Tsang, Levente Karaffa

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbial cell factories, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Alexandra MártonDepartment of Biochemical Engineering, Faculty of Science and Technology, University of Debrecen, Debrecen, Hungary.
Josee VandalCentre for Structural and Functional Genomics, Concordia University, Montreal, QC, Canada.
Vivien BíróDepartment of Biochemical Engineering, Faculty of Science and Technology, University of Debrecen, Debrecen, Hungary.
Erzsébet FeketeDepartment of Biochemical Engineering, Faculty of Science and Technology, University of Debrecen, Debrecen, Hungary. kicsizsoka@yahoo.com.
Erzsébet SándorInstitute of Plant Protection, Faculty of Agricultural and Food Science and Environmental Management, University of Debrecen, Debrecen, Hungary.
Béla KovácsInstitute of Plant Protection, Faculty of Agricultural and Food Science and Environmental Management, University of Debrecen, Debrecen, Hungary.
Adrian TsangCentre for Structural and Functional Genomics, Concordia University, Montreal, QC, Canada.
Levente KaraffaDepartment of Biochemical Engineering, Faculty of Science and Technology, University of Debrecen, Debrecen, Hungary.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundCitric acid, a high-volume bioproduct, is manufactured by submerged fermentation using the filamentous fungus Aspergillus niger. Citric acid overflow requires Mn(II) ion concentrations not exceeding 5 µg L

resultsAspergillus niger citrate fermentations were evaluated across varied manganese profiles, comparing a novel double transporter mutant against parental and single-mutant strains. This strain overexpresses cexA - the primary plasma membrane exporter whose activity overcomes a major production bottleneck - under the control of the strong glaA promoter in a background deficient in dmtA, which encodes the primary high-affinity NRAMP-family manganese transporter. Under high-manganese conditions (100-165 µg L

conclusionsBy demonstrating the maintenance of high-yield overflow metabolism under manganese-replete conditions, alongside enhanced productivity through shortened fermentation times across different vessel scales, this study establishes this A. niger mutant as an attractive cell factory for industrial applications.

Indexed as

Aspergillus nigerCitric AcidManganeseMembrane Transport ProteinsBiomassBioreactorsFermentationFungal ProteinsMutationCitric AcidFungal ProteinsManganeseMembrane Transport ProteinsAspergillus nigerCitrate transporterCitric acidFermentationManganese(II) ionsManganese transporter

Identifiers

PMID42778925
PMCPMC13602490

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.