Beclin1 Regulates Sertoli Cell Function to Maintain Mouse Spermatogenesis.

Yuqing Cai, Wanzhen Li, Yifeilong He, Zhifeng Zhao, Xinyi Lin, Jing Wang, Yuchuan Luo, Yinghe Qin, Yingjie Wu

Abstract read

In one paragraph

Article in Reproductive sciences (Thousand Oaks, Calif.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Yuqing CaiState Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing, People's Republic of China.

Wanzhen LiState Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing, People's Republic of China.

Yifeilong HeState Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing, People's Republic of China.

Zhifeng ZhaoState Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing, People's Republic of China.

Xinyi LinState Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing, People's Republic of China.

Jing WangState Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing, People's Republic of China.

Yuchuan LuoState Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing, People's Republic of China.

Yinghe QinState Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing, People's Republic of China. qinyinghe@cau.edu.cn.

Yingjie WuState Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing, People's Republic of China. wuyingjie@cau.edu.cn.ORCID http://orcid.org/0000-0003-2675-2625

Funding

Earmarked Fund for Modern Agro-industry Technology Research System CARS-43

6 · The paper itself

Abstract

Besides providing nutrients, Sertoli cells support spermatogenic cells development by maintaining seminiferous epithelial polarity, forming the blood-testis barrier (BTB), and phagocytosing residual bodies. Beclin1, a core component of the autophagy initiation complex, is critical for autophagic flux in many cell types. However, its role in Sertoli cells remains unclear. Here, we generated a Sertoli cell-specific Becn1 knockout (cKO) mouse model and found that 9-week-old male cKO mice exhibited reduced fertility with decreased sperm quality and vacuolated seminiferous tubules, and became completely infertile by 12 weeks of age. These defects were associated with disrupted BTB integrity and reduced expression of Claudin-11 in the testes. Further analysis revealed that loss of Beclin1led to impaired autophagosome formation, evidenced by reduced LC3-II/LC3-I ratio. In summary, these findings demonstrate that Beclin1 in Sertoli cells is vital for spermatogenesis and male fertility.

Indexed as

Beclin-1Sertoli CellsSpermatogenesisAnimalsAutophagyBlood-Testis BarrierClaudinsFertilityInfertility, MaleMaleMiceMice, Inbred C57BLMice, KnockoutBeclin-1Becn1 protein, mouseClaudinsCldn11 protein, mouseAutophagyBeclin1Blood-testis barrierSertoli cellSpermiogenesis

Identifiers

PMID42778873

DOI10.1007/978-981-16-2830-6_2

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.