Evidence map›Paper›PMID 42777352›Full record

ArticlePoultry science2026

Integorated multi-omics reveals the NOS2-metabolite-microbiota regulatory axis underlying feed efficiency in laying hens.

Dan Hao, Huifang Li, Wei Liu, Guangqiang Shang, Yan Sun, Yan Zhou, Jie Wang, Dingguo Cao, Chuanyan Che, Fuwei Li

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Poultry science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Dan HaoPoultry Institute, Shandong Academy of Agricultural Sciences, Ji'nan, 250100, China; Shandong Provincial Key Laboratory of Livestock and Poultry Breeding, Ji'nan, 250100, China; Jinan Key Laboratory of Poultry Germplasm Resources Innovation and Healthy Breeding, Ji'nan, 250100, China.
Huifang LiCollege of Veterinary Medicine, Qingdao Agricultural University, Qingdao, 266109, China.
Wei LiuPoultry Institute, Shandong Academy of Agricultural Sciences, Ji'nan, 250100, China; Shandong Provincial Key Laboratory of Livestock and Poultry Breeding, Ji'nan, 250100, China; Jinan Key Laboratory of Poultry Germplasm Resources Innovation and Healthy Breeding, Ji'nan, 250100, China.
Guangqiang ShangCollege of animal Science, Anhui Science and Technology University, Chuzhou, 233100, China.
Yan SunPoultry Institute, Shandong Academy of Agricultural Sciences, Ji'nan, 250100, China; Shandong Provincial Key Laboratory of Livestock and Poultry Breeding, Ji'nan, 250100, China; Jinan Key Laboratory of Poultry Germplasm Resources Innovation and Healthy Breeding, Ji'nan, 250100, China.
Yan ZhouPoultry Institute, Shandong Academy of Agricultural Sciences, Ji'nan, 250100, China; Shandong Provincial Key Laboratory of Livestock and Poultry Breeding, Ji'nan, 250100, China; Jinan Key Laboratory of Poultry Germplasm Resources Innovation and Healthy Breeding, Ji'nan, 250100, China.
Jie WangPoultry Institute, Shandong Academy of Agricultural Sciences, Ji'nan, 250100, China; Shandong Provincial Key Laboratory of Livestock and Poultry Breeding, Ji'nan, 250100, China; Jinan Key Laboratory of Poultry Germplasm Resources Innovation and Healthy Breeding, Ji'nan, 250100, China.
Dingguo CaoPoultry Institute, Shandong Academy of Agricultural Sciences, Ji'nan, 250100, China; Shandong Provincial Key Laboratory of Livestock and Poultry Breeding, Ji'nan, 250100, China; Jinan Key Laboratory of Poultry Germplasm Resources Innovation and Healthy Breeding, Ji'nan, 250100, China.
Chuanyan CheCollege of animal Science, Anhui Science and Technology University, Chuzhou, 233100, China.
Fuwei LiPoultry Institute, Shandong Academy of Agricultural Sciences, Ji'nan, 250100, China; Shandong Provincial Key Laboratory of Livestock and Poultry Breeding, Ji'nan, 250100, China; Jinan Key Laboratory of Poultry Germplasm Resources Innovation and Healthy Breeding, Ji'nan, 250100, China. Electronic address: lifuwei1224@163.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Feed efficiency (Feed conversion ratio, FCR), a critical economic indicator for poultry breeding, is closely associated with the sustainable advancement of the poultry production. However, the molecular mechanisms governing differences in FCR among chickens have not yet been fully elucidated. Transcriptome analysis identified 189 differentially expressed genes, among which NOS2 was identified as a key gene significantly involved in the arginine biosynthesis pathway. Following NOS2 interference for 48 h in primary hepatocytes, CAT, Nrf2, FASN were downregulated, while overexpressed NOS2 showed an opposite tendency. Metabolomics analysis identified 116 and 87 differential metabolites in the liver and cecum contents, respectively. Metagenomics analysis identified s__Methanocorpusculum_vombati as the dominant taxa for LFCR. Integrated analysis revealed that NOS2 expression was positively correlated with 23 liver metabolites. Combined analysis found that genus Oscillibacter as a central hub microbiota, significantly connecting to 2-aminobicyclo[3.1.0]hexane-2,6-dicarboxylic acid in the cecum content. This study elucidates the regulatory role of NOS2 to antioxidant, lipogenesis and inflammatory of chicken hepatocyte, and predicted that NOS2 gene as a central regulator to improve feed efficiency in laying hens.

Indexed as

Feed conversion ratioLaying henMetabolomicsMetagenomicsTranscriptomics

Identifiers

PMID42777352
PMCPMC13634741

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