Evidence map›Paper›PMID 42776250›Full record

ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2026

An integrated molecular cytogenetic platform enables precise characterization of Thinopyrum ponticum chromatin and its structural modifications in wheat background.

Chengzhi Jiang, Min Wan, Jun Wang, Yimin Ren, Ennian Yang, Guangrong Li, Peng Zhang, Zujun Yang

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Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Chengzhi JiangSchool of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 611731, China.
Min WanSchool of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 611731, China.
Jun WangSchool of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 611731, China.
Yimin RenSchool of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 611731, China.
Ennian YangCrop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, China.
Guangrong LiSchool of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 611731, China.
Peng ZhangSchool of Life and Environmental Sciences, Plant Breeding Institute, The University of Sydney, Cobbitty, NSW, 2570, Australia. peng.zhang@sydney.edu.au.
Zujun YangSchool of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 611731, China. yangzujun@uestc.edu.cn.ORCID https://orcid.org/0000-0003-4617-1422

Funding

International Cooperation Project of the Science and Technology Department of Sichuan, China 2022YFH0012National Natural Science Foundation of China 31971886Open Fund of the State Key Laboratory of Wheat Improvement from Shandong Agricultural University WKIF202508)
6 · The paper itself

Abstract

key messageBy integrating ND‑FISH, Oligo‑FISH painting, and chromosome microdissection, we established a comprehensive platform for decaploid Thinopyrum ponticum, enabling karyotyping, sequencing, and marker development, which provides precise tools for the targeted introgression and utilization of alien chromatin in wheat breeding. Thinopyrum ponticum (Podp.) Barkworth & D.R. Dewey, a decaploid wild relative of wheat, represents a valuable genetic reservoir for improving abiotic stress tolerance and disease resistance in wheat breeding. However, its complex genome structure and polyploid nature have long impeded both precise genome assembly and comprehensive characterization of individual chromosomes for wheat-Thinopyrum introgressions. In the present study, we established a reference karyotype of individual Th. ponticum chromosomes by integrated cytogenetic methods combining non‑denaturing fluorescence in situ hybridization (ND‑FISH) and bulked oligonucleotide pool‑based FISH (Oligo‑FISH) painting. This platform was subsequently applied to cytogenetically analyze the wheat-Th. ponticum partial amphiploid 'Agrotana'. Immunostaining assays revealed that Thinopyrum chromosomes maintain normal centromeric histone localization and exhibit distinct methylation patterns in the wheat background. The group-6 chromosomes of the Thinopyrum species harbor several superior resistance genes, we performed microdissection and sequencing of Th. ponticum 6J

Indexed as

ChromatinPoaceaeTriticumChromosomes, PlantGenome, PlantIn Situ Hybridization, FluorescenceKaryotypeKaryotypingPlant BreedingChromatin

Identifiers

PMID42776250

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