ArticleNew microbes and new infections2026
Development and clinical validation of a colorimetric isothermal assay for rapid detection of monkeypox virus.
Article in New microbes and new infections, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: Monkeypox virus has re-emerged as a global public health concern, highlighting the need for rapid, accurate, and accessible diagnostic tools. Real-time quantitative polymerase chain reaction is the reference standard for MPXV detection; however, its reliance on specialized infrastructure limits use in decentralized and resource-limited settings. This study reports the development and clinical evaluation of a colorimetric isothermal amplification assay for the rapid MPXV detection. Methods: A loop-mediated isothermal amplification assay targeting the Orthopoxvirus genus-specific B6R gene and MPXV-specific F3L gene was developed, with human β-actin as an internal control. Reactions were performed at 65 °C for 40 min and interpreted by visual color change. Clinical performance was evaluated using 130 specimens. Analytical specificity was assessed using Vaccinia virus, Buffalopox virus, Herpes simplex virus, and Varicella zoster virus. qPCR served as the reference standard. Results: In this retrospective single-centre evaluation, the assay showed complete concordance with qPCR, correctly identifying all 50 qPCR-positive and 80 qPCR-negative specimens. Detection was achieved across the observed Ct-value range; however, a formal analytical limit of detection was not established. No cross-reactivity was observed with non-Orthopox viruses, while expected differentiation from other Orthopoxviruses was achieved. Conclusions: The developed colorimetric isothermal assay provides a rapid, accurate, and equipment-minimal approach for MPXV detection. The assay demonstrated performance comparable to qPCR within the evaluated clinical panel, supporting its potential utility for diagnosis and surveillance, particularly in settings with limited molecular diagnostic capacity. Further validation in larger prospective multicentre studies is required to confirm its clinical utility across diverse settings.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.