Evidence map›Paper›PMID 42774884›Full record

ArticleiScience2026

Direct detection and quantification of HIV particles and their envelope trimers bound by combinations of broadly neutralizing antibodies.

Souradip Dasgupta, Aswin T Srivatsav, Sameer Kumar, George K Lewis, Anthony L DeVico, Krishanu Ray

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Souradip DasguptaInstitute of Human Virology, University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, MD 21201, USA.
Aswin T SrivatsavInstitute of Human Virology, University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, MD 21201, USA.
Sameer KumarInstitute of Human Virology, University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, MD 21201, USA.
George K LewisInstitute of Human Virology, University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, MD 21201, USA.
Anthony L DeVicoInstitute of Human Virology, University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, MD 21201, USA.
Krishanu RayInstitute of Human Virology, University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, MD 21201, USA.

Funding

Structure-Function Analytics CoreP01AI162242 · NIAID · DUKE UNIVERSITY · PI TOMARAS, GEORGIA DORIS · 2021 to 2025
$22.2M
Detection Assays for Virion Susceptibility to HIV Broadly Neutralizing Antibodies in Plasma and Culture FluidsR61AI176561 · NIAID · UNIVERSITY OF MARYLAND BALTIMORE · PI LEWIS, GEORGE K, RAY, KRISHANU · 2023 to 2025
$1.6M
Broadly neutralizing antibody combinations with single virions in HIV+ plasmaR01AI172487 · NIAID · UNIVERSITY OF MARYLAND BALTIMORE · PI Krishanu Ray · 2023 to 2026
$1.3M
Detection Assays for Virion Susceptibility to HIV Broadly Neutralizing Antibodies in Plasma and Culture FluidsR33AI176561 · NIAID · UNIVERSITY OF MARYLAND BALTIMORE · PI George K Lewis, Krishanu Ray · 2026 to 2026
$654k
NIAID NIH HHS P01 AI162242NIAID NIH HHS R01 AI172487NIAID NIH HHS R33 AI176561NIAID NIH HHS R61 AI176561
6 · The paper itself

Abstract

Broadly HIV-neutralizing antibodies (bnAbs) targeting viral envelope (Env) antigens are promising for HIV/AIDS treatment, functional cure, and prevention. However, individual bnAb epitope classes show distinct gaps in coverage of Env diversity, supporting the use of antibody combinations to improve breadth and potency. We examined how frequently multiple bnAb classes bind simultaneously to individual virions using quantitative single-particle fluorescence correlation spectroscopy (FCS) and FRET approaches. These methods quantified unmodified virion subpopulations from multiple strains bound by one or more fluorescently labeled bnAbs. Analysis of N49P7, PGT121, and PGDM1400, targeting the CD4-binding site, V3-glycan region, and trimer apex, respectively, revealed subpopulations bound by one, two, or three bnAbs. Across strains, the proportion of virions bound by all three antibodies correlated significantly with neutralization activity. These findings define the distribution of combinatorial bnAb binding across heterogeneous virion populations and support quantitative single-particle fluorescence approaches for evaluating antibody combinations against HIV.

Indexed as

bnAbsbroadly neutralizing antibodiescombinatorial antibody bindingEnvFCSfluorescence correlation spectroscopy;förster resonance energy transferFRETHIV-1 envelopesingle-virion quantificationtriple-color coincidence analysis

Identifiers

PMID42774884
PMCPMC13594749

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.