Evidence map›Paper›PMID 42774848›Full record

ArticleiScience2026

SARS-CoV-2 spike-armed vesicular stomatitis virus recapitulates severe COVID-19 acute respiratory distress syndrome in mice.

Prarthna Karunamurthy, Alvin Tieu, Damian Chwastek, Zoe A Fisk, Nikesh Chander, Forough Jahandideh, Doreen Engelberts, Taylor Jamieson, Julia Petryk, Xiaohong He and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Prarthna KarunamurthyDepartment of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada.
Alvin TieuDepartment of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada.
Damian ChwastekDepartment of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada.
Zoe A FiskAcute Care Research Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada.
Nikesh ChanderDepartment of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada.
Forough JahandidehAcute Care Research Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada.
Doreen EngelbertsAcute Care Research Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada.
Taylor JamiesonDepartment of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, ON, Canada.
Julia PetrykDepartment of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, ON, Canada.
Xiaohong HeDepartment of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, ON, Canada.
Carolina IlkowDepartment of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, ON, Canada.
Joanna PoutouDepartment of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, ON, Canada.
Casey LansdellDepartment of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada.
Duncan J StewartDepartment of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada.
Manoj M LaluDepartment of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Clinically relevant preclinical models are critical for understanding acute respiratory distress syndrome (ARDS) and developing novel therapeutics. Although SARS-CoV-2 pseudovirus-based models overcome biosafety challenges associated with live coronavirus, their ability to recapitulate key ARDS features and respond to established treatment remains unclear. We developed a vesicular stomatitis virus expressing the SARS-CoV-2 spike protein (VSV-CoV2-S) for safe use in BSL-2 facilities. We evaluated its ability to induce acute lung injury (ALI) and its responsiveness to dexamethasone. Intratracheal inoculation of K18-hACE2 transgenic mice induced severe ALI characterized by alveolar damage, pulmonary hemorrhage, vascular hyperpermeability, inflammatory cytokine production, and immune cell infiltration. All inoculated transgenic mice died by day 6. Inoculated wild-type mice remained healthy. Dexamethasone attenuated clinical and histopathological ALI evidence in transgenic mice. This study confirms the translational validity of a pseudovirus-based approach, providing a robust and accessible platform to assess ALI pathophysiology and evaluate candidate therapies.

Indexed as

acute lung injuryacute respiratory distress syndromeCOVID-19dexamethasonepseudovirus

Identifiers

PMID42774848
PMCPMC13594746

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.