ArticleFrontiers in microbiology2026
Development and clinical application of a TaqMan-based real-time quantitative PCR assay for rapid detection of goose adenovirus type 4.
Article in Frontiers in microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Introduction: Goose adenovirus type 4 (GoAdV-4) is an emerging pathogen that causes inclusion body hepatitis and hepatic necrosis in goslings, with mortality rates reaching up to 80% in severe outbreaks. Since its first identification in China in 2022, GoAdV-4 has spread rapidly across major goose-producing provinces, posing a serious threat to the domestic goose industry. However, no sensitive and specific quantitative assay has been available for the rapid diagnosis and viral load monitoring of GoAdV-4. Methods: We developed a TaqMan-based real-time quantitative PCR (qPCR) assay targeting the hexon gene of GoAdV-4 and systematically validated its analytical sensitivity, specificity, and repeatability. The assay was then applied to 582 clinical samples of five specimen types (tissues, blood, goose embryos, and cloacal swabs), and its diagnostic performance was compared with conventional PCR. Results: The assay demonstrated a linear detection range of 6.4 × 10 Discussion: The TaqMan qPCR assay detected seven additional positive samples missed by conventional PCR, all confirmed as true positives by Sanger sequencing, demonstrating superior sensitivity. These results show that the established TaqMan qPCR assay is a rapid, sensitive, specific, and reproducible tool for GoAdV-4 detection, providing a valuable diagnostic instrument for the surveillance and control of this emerging waterfowl pathogen.
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