Anti-Allergic Potentials of Actinodaphne angustifolia: Integrated In Vivo Evaluation and In Silico Analysis of Bioactive Compounds.

Actinodaphne angustifolia (Lauraceae), traditionally used for treating inflammatory and allergic disorders was investigated for its anti-allergic potential using integrated in vivo and in silico approaches. Safety evaluation in accordance with OECD guidelines confirmed the non-toxic nature of the ethanolic leaf extract. In a toluene 2,4-diisocyanate (TDI)-induced allergic model, the extract reduced allergic symptoms, including sneezing, nasal scratching, and nasal scores, also normalizing elevated white blood cell counts in blood and bronchoalveolar lavage fluid. A marked reduction in serum IgE levels further indicated suppression of hypersensitivity responses, with effects comparable to cetirizine. HPLC analysis revealed a rich polyphenolic profile dominated by (-) epicatechin and rutin hydrate, while GC-MS identified 15 additional bioactive constituents. Molecular docking demonstrated strong binding affinities of these compounds toward key allergic targets, namely histamine H1 receptor, interleukin-4, and phospholipase A2, supporting their mechanistic roles in anti-allergic activity. The findings validate the ethnomedicinal relevance of A. angustifolia and highlight its potential as a source of plant-derived anti-allergic agents.