Evidence map›Paper›PMID 42773355›Full record

ArticleMetabolomics : Official journal of the Metabolomic Society2026

Metabolic profiling analysis of cytochrome b

Thanyaporn Tengsuttiwat, Adam Burke, Naheed Nazly Kaderbhai, Joe Gallagher, Howbeer Muhamadali, Royston Goodacre

Abstract read
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In one paragraph

Article in Metabolomics : Official journal of the Metabolomic Society, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Thanyaporn TengsuttiwatDepartment of Biochemistry, Cell and Systems Biology, Centre for Metabolomics Research, Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool, L69 7ZB, UK.
Adam BurkeDepartment of Biochemistry, Cell and Systems Biology, Centre for Metabolomics Research, Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool, L69 7ZB, UK.
Naheed Nazly KaderbhaiInstitute of Biological, Environmental and Rural Sciences, Aberystwyth University, Gogerddan Campus, Penglais, Aberystwyth, Ceredigion, SY23 3FL, UK.
Joe GallagherInstitute of Biological, Environmental and Rural Sciences, Aberystwyth University, Gogerddan Campus, Penglais, Aberystwyth, Ceredigion, SY23 3FL, UK.
Howbeer MuhamadaliDepartment of Biochemistry, Cell and Systems Biology, Centre for Metabolomics Research, Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool, L69 7ZB, UK.
Royston GoodacreDepartment of Biochemistry, Cell and Systems Biology, Centre for Metabolomics Research, Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool, L69 7ZB, UK. roy.goodacre@liverpool.ac.uk.

Funding

UKRI (Research Councils) BB/W019558/1
6 · The paper itself

Abstract

introductionRecombinant protein production is pivotal across diverse industries, necessitating efforts to enhance both quantity and quality.

objectivesWe employed gas chromatography coupled with mass spectrometry (GC-MS) to investigate the metabolic effects of producing mammalian cytochrome b

methodsMetabolic profiling involved GC-MS analysis with quality assessments using pooled QCs and multivariate chemometric analysis. This approach revealed specific metabolic features (identified to Level 2 of the Metabolomics Standards Initiative ) correlating significantly with different cultivation conditions and variable copy numbers of the cyt b

resultsOur study shows that CYT b

conclusionOverall, these findings demonstrate that CYT b

Indexed as

Cytochromes b5Escherichia coliGas Chromatography-Mass SpectrometryMetabolomeMetabolomicsRecombinant ProteinsCytochromes b5Recombinant ProteinsChemometricsCytochrome b 5GC-MSMetabolic profileRecombinant protein production

Identifiers

PMID42773355
PMCPMC13597647

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.