Evidence map›Paper›PMID 42772279›Full record

ArticleCell2026

A geothermal amoeba sets a new upper temperature limit for eukaryotes.

H Beryl Rappaport, Natalie A Petek-Seoane, Tomáš Tyml, Jessica K Niblo, Felix Mikus, Naomi E Gilbert, Kurt LaButti, Godwin Ani, Ethan MacVicar, Rachel M Shepherd and 12 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

22 authors.

H Beryl RappaportBiology Department, Syracuse University, Syracuse, NY, USA; DOE Joint Genome Institute, Berkeley, CA, USA. Electronic address: hbrappap@syr.edu.
Natalie A Petek-SeoaneDepartment of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA, USA.
Tomáš TymlDOE Joint Genome Institute, Berkeley, CA, USA.
Jessica K NibloDepartment of Chemistry, Syracuse University, Syracuse, NY, USA.
Felix MikusDepartment of Biochemistry, University of Geneva, Geneva, Switzerland.
Naomi E GilbertBiology Department, Syracuse University, Syracuse, NY, USA.
Kurt LaButtiDOE Joint Genome Institute, Berkeley, CA, USA.
Godwin AniDepartment of Biological Sciences, Smith College, Northampton, MA, USA; Program in Organismic and Evolutionary Biology, University of Massachusetts-Amherst, Amherst, MA, USA.
Ethan MacVicarDepartment of Biology, Gonzaga University, Spokane, WA, USA.
Rachel M ShepherdBiology Department, Syracuse University, Syracuse, NY, USA.
Ignacio de la HigueraCenter for Life in Extreme Environments, Department of Biology, Portland State University, Portland, OR, USA.
Samuel J LordDepartment of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA, USA.
Gautam DeyCell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
Gordon V WolfeDepartment of Biological Sciences, California State University, Chico, CA, USA.
Omaya DudinDepartment of Biochemistry, University of Geneva, Geneva, Switzerland.
Laura A KatzDepartment of Biological Sciences, Smith College, Northampton, MA, USA; Program in Organismic and Evolutionary Biology, University of Massachusetts-Amherst, Amherst, MA, USA.
Kenneth M StedmanCenter for Life in Extreme Environments, Department of Biology, Portland State University, Portland, OR, USA.
Shahar SukenikBiology Department, Syracuse University, Syracuse, NY, USA; Department of Chemistry, Syracuse University, Syracuse, NY, USA.
Kristen SkruberDepartment of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA, USA; Department of Biology, Gonzaga University, Spokane, WA, USA.
Frederik SchulzDOE Joint Genome Institute, Berkeley, CA, USA.
R Dyche MullinsDepartment of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA, USA.
Angela M OliverioBiology Department, Syracuse University, Syracuse, NY, USA. Electronic address: amoliver@syr.edu.

Funding

Assembly and function of cytoskeletal systems in eukaryotic and prokaryoticcellsR35GM118119 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI MULLINS, R DYCHE · 2016 to 2025
$4.8M
Uncovering the structural underpinnings of function in disordered transcription factor regionsR35GM137926 · NIGMS · UNIVERSITY OF CALIFORNIA, MERCED · PI Shahar Sukenik · 2020 to 2026
$2.5M
Determining the molecular basis for extreme virus stability, a structure-guided approachR16GM154184 · NIGMS · PORTLAND STATE UNIVERSITY · PI Kenneth Mark Stedman · 2024 to 2026
$448k
Identifying fundamental principles of evolutionary dynamics in multispecies microbiomesR35GM162848 · NIGMS · SYRACUSE UNIVERSITY · PI Angela M Oliverio · 2026 to 2026
$411k
NIGMS NIH HHS R16 GM154184NIGMS NIH HHS R35 GM118119NIGMS NIH HHS R35 GM137926NIGMS NIH HHS R35 GM162848
6 · The paper itself

Abstract

The study of temperature limits has transformed our understanding of life's boundaries but has focused on bacteria and archaea. For decades after their discovery, no eukaryote was shown to replicate above 60°C. We isolated a geothermal amoeba, Incendiamoeba cascadensis, that divides at 63°C and is motile up to 64°C. We identified an enrichment of genes related to proteostasis and environmental sensing relative to mesophilic amoebae. Comparative RNA sequencing of I. cascadensis revealed upregulation of pathways related to proteostasis, DNA repair, and membrane trafficking at high temperatures. Predicted proteins of I. cascadensis have distinct biophysical properties, including enrichment of positively charged surface residues similar to bacterial and archaeal hyperthermophiles, which suggests a convergent mechanism for protein stability under temperature stress. Together, our findings expand our understanding of where and how life can persist.

Indexed as

AmoebaHot TemperatureProtozoan ProteinsTemperatureProtozoan Proteinsamoebaeukaryotic cellextremophilegeothermalprotistthermophile

Identifiers

PMID42772279
PMCPMC13598948

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.