Evidence map›Paper›PMID 42771319›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2027

Virus-Like Particles as a Nonviral CRISPR/Cas Editing Delivery Tool.

Antonio Carusillo, Asma Naseem, Alessia Cavazza

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2027. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Antonio CarusilloCenter for Genomic Medicine and Rare Diseases, Department of Medical and Surgical Sciences for Children and Adults, University of Modena and Reggio Emilia, Modena, Italy.
Asma NaseemInfection, Immunity & Inflammation Research, and Teaching Department, UCL Great Ormond Street Institute of Child Health, University College London, 20 Guilford Street, London, UK.
Alessia CavazzaCenter for Genomic Medicine and Rare Diseases, Department of Medical and Surgical Sciences for Children and Adults, University of Modena and Reggio Emilia, Modena, Italy. alessia.cavazza@unimore.it.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Virus-like particles (VLPs) are self-assembling viral protein complexes that mimic native virions while lacking replicative and infectious capacity. By retaining efficient cellular entry and cargo protection, they overcome key limitations of viral vectors, including insertional mutagenesis and strong immunogenicity. Their ease of production, flexible cargo capacity, and tunable tropism make them highly versatile tools. Here, we describe a protocol for generating VSV-G-pseudotyped VLPs that deliver CRISPR/Cas9 RNPs and their functional validation in surrogate cell lines.

Indexed as

CRISPR-Cas SystemsGene EditingGene Transfer TechniquesVirionGenetic VectorsHEK293 CellsHumansMembrane GlycoproteinsViral Envelope ProteinsG protein, vesicular stomatitis virusMembrane GlycoproteinsViral Envelope ProteinsCRISPR/Cas9Gene editingGenome editingNonviral deliveryNucleic acid deliveryRNP deliveryVirus-like particles (VLPs)VSV-G pseudotyping

Identifiers

PMID42771319

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.