Evidence map›Paper›PMID 42771249›Full record

ArticleMolecular biology reports2026

Identification and functional annotation of drought-induced molecular pathways in sugarcane using suppression subtractive hybridization.

Lakshmi Kasirajan, Rachel Lissy Vargheese, Indusha Yazhini Sankararaj, Sheelamary Sebastiar, Suganya Angannan, Annadurai Ayyadurai, Rabisha Valiyaparambth, Akila Dharshini Venkatachalam

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Article in Molecular biology reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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8 authors.

Lakshmi KasirajanDivision of Crop Improvement, ICAR-Sugarcane Breeding Institute, Coimbatore, 641007, Tamil Nadu, India. lakshmimbb@gmail.com.ORCID http://orcid.org/0000-0001-6620-9841
Rachel Lissy VargheeseDivision of Crop Improvement, ICAR-Sugarcane Breeding Institute, Coimbatore, 641007, Tamil Nadu, India.
Indusha Yazhini SankararajDivision of Crop Improvement, ICAR-Sugarcane Breeding Institute, Coimbatore, 641007, Tamil Nadu, India.
Sheelamary SebastiarDivision of Crop Improvement, ICAR-Sugarcane Breeding Institute, Coimbatore, 641007, Tamil Nadu, India.
Suganya AngannanDivision of Crop Improvement, ICAR-Sugarcane Breeding Institute, Coimbatore, 641007, Tamil Nadu, India.
Annadurai AyyaduraiDivision of Crop Improvement, ICAR-Sugarcane Breeding Institute, Coimbatore, 641007, Tamil Nadu, India.
Rabisha ValiyaparambthDivision of Crop Improvement, ICAR-Sugarcane Breeding Institute, Coimbatore, 641007, Tamil Nadu, India.
Akila Dharshini VenkatachalamDivision of Crop Improvement, ICAR-Sugarcane Breeding Institute, Coimbatore, 641007, Tamil Nadu, India.

Funding

Indian Council of Agricultural Research P1-15/1-3(X)
6 · The paper itself

Abstract

backgroundWild ancestors of sugarcane, in particular Saccharum spontaneum, are inherently tolerant to abiotic stress. Interspecific hybrids derived from S. spontaneum possess agronomically desirable traits in addition to their potential for sustaining themselves in drought-stressed conditions. METHODS AND

resultsIn our study, suppression subtractive hybridisation was used to identify drought-responsive candidate transcripts (DRCTs) under water deficit conditions in AS 04-1687 and TS-GS 2024, which were validated by physiological and molecular expression analysis. The amplicon size of the subtraction libraries ranged from 200 bp to 1.6 kb for AS 04-1687, whereas for TS-GS 20-24, it ranged from 250 bp to 1.1 kb. The sequenced raw reads were processed, assembled, and functionally annotated. A total of 452 and 625 differentially expressed drought-induced transcripts were identified in AS 04-1687 and TSGS 20-24, respectively. Functional annotation revealed that the expression of a substantial portion of these transcripts was associated with protein modification. In AS 04-1687, drought tolerance was associated with coumarin-mediated stress responses, whereas in TSGS 20-24, drought response was regulated by ABA-mediated signalling cascade and the ascorbate biosynthetic pathway.

conclusionTranscripts belonging to the MYB, NAC, bZIP, SPL, and WRKY were identified as key regulators of drought stress in both genotypes, while in TSGS 20-24, additional transcriptional regulation via JAZ families contributed to coordinating plant defence via ABA signalling, osmotic adjustment, and physiological remodelling. These findings offer valuable insights for identifying novel genes that enhance drought resistance in sugarcane.

Indexed as

SaccharumDrought ResistanceDroughtsGene Expression ProfilingGene Expression Regulation, PlantMolecular Sequence AnnotationPlant ProteinsSignal TransductionStress, PhysiologicalSubtractive Hybridization TechniquesPlant ProteinsDifferentially expressed transcriptsDrought toleranceInterspecific hybridsSaccharum spontaneumSuppression Subtractive Hybridisation

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PMID42771249

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