Evidence map›Paper›PMID 42771243›Full record

ArticleBulletin of experimental biology and medicine2026

Expression of the Thyroglobulin ChEL Domain in Whole Blood of Patients with Thyroid Pathology.

A V Zubkov, I V Zubkova, O A Svitich, V V Fadeev, L G Butova

Abstract read
PubMed Publisher
In one paragraph

Article in Bulletin of experimental biology and medicine, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

A V ZubkovI. Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia. alex_zubkov@list.ru.
I V ZubkovaSechenov First Moscow State Medical University, Ministry of Health of the Russian Federation (Sechenov University), Moscow, Russia.
O A SvitichI. Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia.
V V FadeevSechenov First Moscow State Medical University, Ministry of Health of the Russian Federation (Sechenov University), Moscow, Russia.
L G ButovaI. Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The expression of the thyroglobulin (Tg) cholinesterase-like domain (ChEL domain) was detected in whole blood samples from a patient with Graves' disease and a healthy donor. cDNA templates of the TG ChEL domain were synthesized from mRNA isolated from these samples. Using three of the six primer pairs synthesized to amplify human TG cDNA fragments targeting the ChEL domain, four PCR products were obtained and cloned into the pAL2‑T vector. Using PCR and Sanger sequencing, a 900‑bp TG ChEL domain cDNA fragment with three nucleotide substitutions (c.7408C>T, c.7501T>C, and c.7589G>A) was detected in the healthy donor genome. The 900‑bp cDNA fragment from the patient with Graves' disease harbored a single nucleotide substitution c.8071C>T. The expression of these fragments in eukaryotic cell lines may serve as the basis for the production of recombinant Tg peptides in test systems that allow for the comprehensive diagnosis of various thyroid diseases.

Indexed as

mutations in the TG generecombinant vectorTG ChEL domainthyroglobulinthyroid autoantigens

Identifiers

PMID42771243

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.