Evidence map›Paper›PMID 42771149›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2027

Analysis of Chlamydia trachomatis Gene Function via Controlled Up- or Downregulation.

Colleen C Monahan, Nicole A Grieshaber, Scott S Grieshaber, Anders Omsland

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2027. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Colleen C MonahanPaul G. Allen School for Global Health, Washington State University, Pullman, WA, 99164, USA.
Nicole A GrieshaberDepartment of Biological Sciences, University of Idaho, Moscow, ID, 83844, USA. ngrieshaber@uidaho.edu.
Scott S GrieshaberDepartment of Biological Sciences, University of Idaho, Moscow, ID, 83844, USA.
Anders OmslandPaul G. Allen School for Global Health, Washington State University, Pullman, WA, 99164, USA. anders.omsland@wsu.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Due to genome streamlining, many genes in the ~1 Mb genome of Chlamydia trachomatis are essential and cannot be deleted. Ectopic overexpression and CRISPRi-based knockdown are complementary genetic approaches to study the function of genes in C. trachomatis, including essential genes. When used in combination with inducible promoters, these tools allow researchers to determine how precise regulation of gene expression contributes to proper progression of the chlamydial developmental cycle. This chapter includes protocols which detail ectopic overexpression of a protein or conversely, transcriptional inhibition of a specific gene in C. trachomatis serovar L2/434/Bu.

Indexed as

Chlamydia trachomatisGene Expression Regulation, BacterialBacterial ProteinsChlamydia InfectionsDown-RegulationGene Knockdown TechniquesGenes, EssentialHumansPromoter Regions, GeneticUp-RegulationBacterial ProteinsCRISPRi-based knockdownDevelopmental transitionsDose–response analysisEctopic overexpressionEssential genes

Identifiers

PMID42771149

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.