Evidence map›Paper›PMID 42771139›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2027

Chlamydia trachomatis Infection in a Microphysiologic Cell Culture Model.

David J Delgado Diaz, Katherine M Nelson, Vonetta L Edwards, Ian J Glomski, Filipa Ribeiro, Patrik M Bavoil, Alison K Criss, Jacques Ravel, Jason P Gleghorn, Isabelle Derré

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2027. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

David J Delgado Diaz *Department of Microbiology, Immunology, and Cancer Biology, University of Virginia, Charlottesville, VA, USA.
Katherine M Nelson *Department of Biomedical Engineering, University of Delaware, Newark, DE, USA.
Vonetta L EdwardsCenter for Advanced Microbiome Research and Innovation, Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD, USA.
Ian J GlomskiDepartment of Microbiology, Immunology, and Cancer Biology, University of Virginia, Charlottesville, VA, USA.
Filipa RibeiroDepartment of Biomedical Engineering, University of Delaware, Newark, DE, USA.
Patrik M BavoilDepartment of Microbial Pathogenesis, University of Maryland School of Medicine, Baltimore, MD, USA.
Alison K CrissDepartment of Microbiology, Immunology, and Cancer Biology, University of Virginia, Charlottesville, VA, USA.
Jacques RavelCenter for Advanced Microbiome Research and Innovation, Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD, USA.
Jason P GleghornDepartment of Biomedical Engineering, University of Delaware, Newark, DE, USA. gleghorn@udel.edu.
Isabelle DerréDepartment of Microbiology, Immunology, and Cancer Biology, University of Virginia, Charlottesville, VA, USA. id8m@virgina.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Chlamydia trachomatis is an obligate intracellular bacterium that infects the columnar epithelium of the human endocervix. While conventional two-dimensional cell cultures and animal models have been instrumental in advancing our understanding of C. trachomatis biology, they are limited in capturing the multicellularity, architecture, and physiological microenvironment of the human cervix. This chapter describes the use of a three-dimensional (3D) microphysiologic model to study Chlamydia trachomatis infection. The model is inexpensively made without specialized equipment and is designed to recreate the epithelial-stromal interface. We outline procedures for coculturing cervical epithelial cells and fibroblasts, infecting epithelial cells with fluorescently labeled C. trachomatis, monitoring infection progression via fluorescent microscopy, and quantifying infectious progeny. The complete developmental cycle of C. trachomatis within this model provides a robust and accessible platform to investigate C. trachomatis-specific host-pathogen interactions, immune responses, and the influence of diverse physiological and environmental stimuli within a relevant cervical context.

Indexed as

Chlamydia InfectionsChlamydia trachomatisCell Culture TechniquesCervix UteriCoculture TechniquesEpithelial CellsFemaleFibroblastsHeLa CellsHost-Pathogen InteractionsHumansMicrophysiological SystemsMicroscopy, FluorescenceBiomimeticCervical microenvironmentChlamydia trachomatisEpithelial cellFibroblastsOrgan-on-a-chipThree-dimensional model

Identifiers

PMID42771139

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.