Evidence map›Paper›PMID 42770015›Full record

ArticleBiomedical optics express2026

Optimal excitation polarization schemes to boost the precision of single-molecule orientation imaging.

Kaizhi A Nie, Yuanxin Qiu, Matthew D Lew

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedical optics express, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Kaizhi A NiePreston M. Green Department of Electrical and Systems Engineering, McKelvey School of Engineering, Washington University in St. Louis, MO 63130, USA.ORCID https://orcid.org/0009-0006-9814-5444
Yuanxin QiuPreston M. Green Department of Electrical and Systems Engineering, McKelvey School of Engineering, Washington University in St. Louis, MO 63130, USA.ORCID https://orcid.org/0009-0008-4548-3057
Matthew D LewPreston M. Green Department of Electrical and Systems Engineering, McKelvey School of Engineering, Washington University in St. Louis, MO 63130, USA.ORCID https://orcid.org/0000-0002-5614-3292

Funding

Six-Dimensional Single-Molecule Nanoscopy for Elucidating the Dynamic Organization of BiomoleculesR35GM124858 · NIGMS · WASHINGTON UNIVERSITY · PI Matthew D Lew · 2017 to 2026
$3.3M
NIGMS NIH HHS R35 GM124858
6 · The paper itself

Abstract

Modulating excitation polarization across camera frames can improve the precision of single-molecule orientation-localization microscopy (SMOLM), but principled methods for designing these excitation sequences remain underdeveloped. Here, we introduce a Fisher-information framework for optimizing linearly and circularly polarized excitation schemes under a fixed total illumination photon budget. For the multi-view reflector microscope, 3000 illumination photons, and 7.5 detected background photons per pixel, an optimized four-frame linear scheme improves median orientation measurement precision by 21.2% relative to the conventional six-frame equal-pumping scheme and achieves Cramér-Rao-bound-limited median precisions of

Identifiers

PMID42770015
PMCPMC13592251

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.