ArticleInternational journal of legal medicine2026
A novel 16-plex DIP-SNP panel for forensic analysis of imbalanced DNA mixtures in a Han Chinese population.
Article in International journal of legal medicine, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
In the field of forensic genetics, the discovery of new genetic markers and the development of rapid detection techniques are of great significance for the identification of biological samples. The compound genetic marker DIP-SNP (deletion/insertion polymorphism-single nucleotide polymorphism) offers distinct advantages, including high polymorphism, the absence of stutter peaks, and a low mutation rate, making it a promising tool for the analysis of DNA mixtures. However, DIP-SNP genotyping based on capillary electrophoresis typically requires two separate amplification reactions, resulting in complex and time-consuming procedures. In this study, sixteen DIP-SNP markers were selected to develop a five-color fluorescent multiplex panel based on the amplification refractory mutation system (ARMS) and fragment length discrepant allele specific polymerase chain reaction (FLDAS-PCR) principles, enabling simultaneous genotyping in a single-tube reaction. In a Han Chinese population from Hubei province (n = 165), the effective number of alleles (A
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