Protocol for spatially restricted CreERT2-mediated recombination in mouse brain-infiltrating B cells using intracerebral 4-hydroxy-tamoxifen.

Mika Schneider, Georgios Perdikaris, Tobias Derfuss, Nicholas S R Sanderson

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In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

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4 · The record

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5 · Who and what money

Authors and funding

4 authors.

Mika SchneiderDepartment of Biomedicine, University Hospital Basel and University of Basel, Basel, Switzerland; Multiple Sclerosis Centre and Research Center for Clinical Neuroimmunology and Neuroscience (RC2NB), University Hospital Basel and University of Basel, Basel, Switzerland. Electronic address: mikaschneider@gmx.ch.

Georgios PerdikarisDepartment of Biomedicine, University Hospital Basel and University of Basel, Basel, Switzerland; Multiple Sclerosis Centre and Research Center for Clinical Neuroimmunology and Neuroscience (RC2NB), University Hospital Basel and University of Basel, Basel, Switzerland.

Tobias DerfussDepartment of Biomedicine, University Hospital Basel and University of Basel, Basel, Switzerland; Multiple Sclerosis Centre and Research Center for Clinical Neuroimmunology and Neuroscience (RC2NB), University Hospital Basel and University of Basel, Basel, Switzerland; Department of Neurology, University Hospital Basel, Basel, Switzerland.

Nicholas S R SandersonDepartment of Biomedicine, University Hospital Basel and University of Basel, Basel, Switzerland; Multiple Sclerosis Centre and Research Center for Clinical Neuroimmunology and Neuroscience (RC2NB), University Hospital Basel and University of Basel, Basel, Switzerland. Electronic address: nicholas.sanderson@unibas.ch.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Tamoxifen-dependent, cre-recombinase-mediated techniques enable precise temporal control of gene expression, but do not always provide sufficient anatomical control. Here, we present a protocol to restrict gene expression using local injection of 4-hydroxy-tamoxifen in bone wax. As an example, we describe the induction of the viral protein LMP1 and the fluorescent protein tdTomato only in brain-infiltrating B cells. We describe steps for intracerebral virus injection and 4-hydroxy-tamoxifen administration in mice. For complete details on the use and execution of this protocol, please refer to Kim et al.

Indexed as

ImmunologyNeuroscienceSystems biology

Identifiers

PMID42766411

DOI10.1016/j.xpro.2026.104856

PMCPMC13629331

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.