Application of Pepper aptamer-based reporter system for bacterial transcription studies.

Feixue Zhang, Yue Wang, Yue Mao, Bingbing Hou, Jiang Ye, Haizhen Wu, Ruida Wang, Huizhan Zhang

Abstract read

In one paragraph

Article in Archives of microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Feixue ZhangState Key Laboratory of Bioreactor Engineering, Department of Applied Biology, Bioengineering Experiment Teaching Demonstration Center, School of Biotechnology, East China University of Science and Technology, Shanghai, 200237, China.

Yue WangState Key Laboratory of Bioreactor Engineering, Department of Applied Biology, Bioengineering Experiment Teaching Demonstration Center, School of Biotechnology, East China University of Science and Technology, Shanghai, 200237, China.

Yue MaoState Key Laboratory of Bioreactor Engineering, Department of Applied Biology, Bioengineering Experiment Teaching Demonstration Center, School of Biotechnology, East China University of Science and Technology, Shanghai, 200237, China.

Bingbing HouShanghai Yaxin Biotechnology Co. Ltd, Shanghai, 201406, China.

Jiang YeState Key Laboratory of Bioreactor Engineering, Department of Applied Biology, Bioengineering Experiment Teaching Demonstration Center, School of Biotechnology, East China University of Science and Technology, Shanghai, 200237, China.

Haizhen WuState Key Laboratory of Bioreactor Engineering, Department of Applied Biology, Bioengineering Experiment Teaching Demonstration Center, School of Biotechnology, East China University of Science and Technology, Shanghai, 200237, China. wuhzh@ecust.edu.cn.ORCID https://orcid.org/0000-0001-8528-9269

Ruida WangState Key Laboratory Incubation Base for Conservation and Utilization of Bio-Resource in Tarim Basin, College of Life Science and Technology, Tarim University, Alar, 843300, China. wrd@taru.edu.cn.

Huizhan ZhangState Key Laboratory of Bioreactor Engineering, Department of Applied Biology, Bioengineering Experiment Teaching Demonstration Center, School of Biotechnology, East China University of Science and Technology, Shanghai, 200237, China.

Funding

National Key Research and Development Program of China 2021YFC2100600National Natural Science Foundation of China 32541085 and 42406126President's Fund of Tarim University TDZKBS202536Xinjiang Leading Talent Introduction Program (Key University Talent Recruitment Project) XJRC-2025-BTJY-YJ-GX-QNQZ-006

6 · The paper itself

Abstract

This study introduces a novel bacterial reporter system using the fluorescent RNA aptamer Pepper to directly monitor mRNA-level transcriptional changes, overcoming limitations of traditional protein-based reporters. First, the Pepper reporter system was used to characterize the strength differences of promoters used in Escherichia coli, and the result were compared with enhanced green fluorescent protein (eGFP) reporter and quantitative real-time PCR (qRT-PCR) analysis. Then the Pepper reporter system was used to examine six pairs of known transcriptional regulatory relationships: RacR activating P

Indexed as

Aptamers, NucleotideGenes, ReporterTranscription, GeneticBacillus subtilisBacterial ProteinsEscherichia coliGene Expression Regulation, BacterialGreen Fluorescent ProteinsPromoter Regions, GeneticAptamers, NucleotideBacterial ProteinsGreen Fluorescent ProteinsGene expressionPepper aptamerReporter systemTranscriptional regulation

Identifiers

PMID42766120

DOI10.1039/c8cc08796b

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.