Evidence map›Paper›PMID 42763861›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2027

Live Fluorescence Lifetime Imaging Microscopy (FLIM) of the Epithelial Polarity and Cell-Specific Segmentation in the Small Intestinal Organoids.

Hang Zhou, Irina A Okkelman, Amber De Kinder, Bert Devriendt, Max Nobis, Ruslan I Dmitriev

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2027. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Hang ZhouTissue Engineering and Biomaterials Group, Department of Human Structure and Repair, Faculty of Medicine and Health Sciences, Ghent University, The Core, C. Heymanslaan 10,, 9000, Ghent, Belgium.
Irina A OkkelmanTissue Engineering and Biomaterials Group, Department of Human Structure and Repair, Faculty of Medicine and Health Sciences, Ghent University, The Core, C. Heymanslaan 10,, 9000, Ghent, Belgium.
Amber De KinderTissue Engineering and Biomaterials Group, Department of Human Structure and Repair, Faculty of Medicine and Health Sciences, Ghent University, The Core, C. Heymanslaan 10,, 9000, Ghent, Belgium.
Bert DevriendtLaboratory of Immunology, Department of Translational Physiology, Infectiology and Public Health, Faculty of Veterinary Medicine, Ghent University,, 9820, Merelbeke, Belgium.
Max NobisIntravital Imaging Expertise Center, VIB Center for Cancer Biology, KU Leuven,, 3000, Leuven, Belgium.
Ruslan I DmitrievTissue Engineering and Biomaterials Group, Department of Human Structure and Repair, Faculty of Medicine and Health Sciences, Ghent University, The Core, C. Heymanslaan 10,, 9000, Ghent, Belgium. Ruslan.dmitriev@ugent.be.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Apical-basal polarity is essential for gastrointestinal epithelium function, where the apical membrane mediates nutrient absorption, host-microbiota interactions, and pathogen defense. However, conventional intestinal organoid culture displays an "apical-in" topology, limiting functional research with respect to the apical membrane. F-actin labeling with fluorescent phalloidin, a well-established apical marker, is also incompatible with live imaging. To address these limitations, in this chapter we describe an extracellular matrix (ECM) removal protocol to achieve "apical-out" topology in porcine intestinal organoid model and further validated WGA and Nile Red live imaging tracers enabling discrimination between apical-out (AO) and basal-out (BO) organoids. Subsequently, using integrated computational pipelines (automatic nuclei segmentation and single-nucleus phasor-based lifetime analysis), we can quantify organoid proliferative states at single-nucleus resolution following BrdU and Hoechst 33342 staining. Using the analysis of Hoechst 33342 fluorescence lifetime, we demonstrate that both organoid topologies exhibit intrinsic proliferative heterogeneity, but overall proliferative capacity is topology-dependent: apical-out organoids displayed decreased proliferation, whereas basal-out organoids remain highly proliferative.

Indexed as

Cell PolarityEpithelial CellsIntestine, SmallOptical ImagingOrganoidsAnimalsImage Processing, Computer-AssistedIntestinal MucosaMicroscopy, FluorescenceSwineCell proliferationFLIMIntestinal organoidLive microscopyPolarity reversion

Identifiers

PMID42763861

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.