Evidence map›Paper›PMID 42763396›Full record

ArticleVeterinary research2026

Mapping of three novel linear B-cell epitopes on the VP7 protein of epizootic hemorrhagic disease virus with monoclonal antibodies.

Xinbing Hu, Yunru Zhong, Yingjuan He, Mingxin Zhang, Fanhua Meng, Yu Zhang, Zhancheng Tian, Guiquan Guan, Jijun He, Youjun Shang and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Veterinary research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Xinbing HuState Key Laboratory of Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, College of Veterinary Medicine, Chinese Academy of Agricultural Sciences, Lanzhou University, Lanzhou, 730000, China.
Yunru ZhongState Key Laboratory of Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, College of Veterinary Medicine, Chinese Academy of Agricultural Sciences, Lanzhou University, Lanzhou, 730000, China.
Yingjuan HeState Key Laboratory of Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, College of Veterinary Medicine, Chinese Academy of Agricultural Sciences, Lanzhou University, Lanzhou, 730000, China.
Mingxin ZhangState Key Laboratory of Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, College of Veterinary Medicine, Chinese Academy of Agricultural Sciences, Lanzhou University, Lanzhou, 730000, China.
Fanhua MengState Key Laboratory of Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, College of Veterinary Medicine, Chinese Academy of Agricultural Sciences, Lanzhou University, Lanzhou, 730000, China.
Yu ZhangState Key Laboratory of Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, College of Veterinary Medicine, Chinese Academy of Agricultural Sciences, Lanzhou University, Lanzhou, 730000, China.
Zhancheng TianState Key Laboratory of Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, College of Veterinary Medicine, Chinese Academy of Agricultural Sciences, Lanzhou University, Lanzhou, 730000, China.
Guiquan GuanState Key Laboratory of Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, College of Veterinary Medicine, Chinese Academy of Agricultural Sciences, Lanzhou University, Lanzhou, 730000, China.
Jijun HeState Key Laboratory of Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, College of Veterinary Medicine, Chinese Academy of Agricultural Sciences, Lanzhou University, Lanzhou, 730000, China.
Youjun ShangState Key Laboratory of Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, College of Veterinary Medicine, Chinese Academy of Agricultural Sciences, Lanzhou University, Lanzhou, 730000, China.
Jun AiAnimal Quarantine Laboratory, Technology Center of Kunming Customs, Kunming, 650200, China. 52563122@qq.com.
Junzheng DuState Key Laboratory of Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, College of Veterinary Medicine, Chinese Academy of Agricultural Sciences, Lanzhou University, Lanzhou, 730000, China. dujunzheng@caas.cn.

Funding

China Agriculture Research System of MARA CARS-39-13China Agriculture Research System of MOF CARS-38Innovation Program of Chinese Academy of Agricultural Sciences CAAS-ASTIP-2021-LVRINational Key Research and Development Program of China 2024YFD1800104
6 · The paper itself

Abstract

Epizootic hemorrhagic disease virus (EHDV) is an important Orbivirus transmitted by culicoides midges. EHDV poses a significant threat to ruminant production worldwide. The VP7 protein is a highly conserved, group-specific antigen of EHDV, which serves as a key target for serological diagnosis. In this study, the recombinant VP7 (r-VP7) protein of EHDV-1 was expressed in an Escherichia coli expression system and used to immunize BALB/c mice. Four hybridoma cell lines secreting monoclonal antibodies (mAbs) against VP7 were successfully generated by the hybridoma technique, and named 5D1, 6A7, 7B11, and 7C4. Indirect ELISA, western blot analysis, and immunofluorescence assays demonstrated that all four mAbs specifically recognized both the r-VP7 protein and the native VP7 protein in EHDV-1-infected BHK-21 cells, with favorable reactivity and specificity. The VP7 protein was progressively truncated and expressed as a series of GST fusion proteins, and the linear B-cell epitopes recognized by these mAbs were precisely identified by western blotting. The results showed that 5D1 and 7B11 recognized the epitope

Indexed as

Antibodies, MonoclonalEpitopes, B-LymphocyteHemorrhagic Disease Virus, EpizooticViral Core ProteinsAnimalsAntibodies, ViralEnzyme-Linked Immunosorbent AssayEpitope MappingFemaleMiceMice, Inbred BALB CReoviridae InfectionsAntibodies, MonoclonalAntibodies, ViralEpitopes, B-LymphocyteViral Core ProteinsVP7 protein, orbivirusB-cell epitope mappingEpizootic hemorrhagic disease virusmonoclonal antibodiesVP7 protein

Identifiers

PMID42763396
PMCPMC13589380

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.