Evidence map›Paper›PMID 42761875›Full record

ArticleMethodsX2026

A lyophilization-compatible colorimetric LAMP assay for cold chain-free, extraction-free and rapid post-enrichment detection of

Wansadaj Jaroenram, Yada Ongchanchai, Prachumporn Nounurai, Supattana Janta, Wansika Kiatpathomchai

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Article in MethodsX, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Wansadaj JaroenramNational Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 111 Thailand Science Park, Phahonyothin Rd., Klong Neung, Klong Luang, Pathum Thani, 12120, Thailand.
Yada OngchanchaiNational Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 111 Thailand Science Park, Phahonyothin Rd., Klong Neung, Klong Luang, Pathum Thani, 12120, Thailand.
Prachumporn NounuraiNational Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 111 Thailand Science Park, Phahonyothin Rd., Klong Neung, Klong Luang, Pathum Thani, 12120, Thailand.
Supattana JantaNational Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 111 Thailand Science Park, Phahonyothin Rd., Klong Neung, Klong Luang, Pathum Thani, 12120, Thailand.
Wansika KiatpathomchaiNational Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 111 Thailand Science Park, Phahonyothin Rd., Klong Neung, Klong Luang, Pathum Thani, 12120, Thailand.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Developing reliable dry-format colorimetric LAMP assays remains challenging because most existing systems depend on pH-sensitive dyes e.g. Hydroxy Naphthol Blue and Phenol Red. These dyes lose performance after lyophilization or when exposed to extraction-free sample buffers, as they require low-buffer conditions to generate accurate color transitions. Lyophilization disrupts pH stability, and common lysis additives further interfere with signal clarity. Consequently, creating a robust, field-ready colorimetric LAMP platform has been constrained. Here, we developed a Mn-PAPS Colorimetric LAMP assay, a metal ion-based colorimetric system that is inherently pH-independent and fully compatible with freeze-drying. Using

Indexed as

Colorimetric LAMPEscherichia coliFoodborne pathogen detectionLyophilized diagnosticsMetal-dye system

Identifiers

PMID42761875
PMCPMC13587809

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.