Evidence map›Paper›PMID 42761597›Full record

ArticlePeerJ2026

Development and analytical validation of a multiplex qPCR assay for

Abdirahman Hussein Elmi, Chan Yean Yean, Nor Rosidah Ibrahim, Manal Abdel Haleem A Abusalah, Zeehaida Mohamed

Abstract readValidation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in PeerJ, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Abdirahman Hussein ElmiDepartment of Medical Microbiology and Parasitology, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, Kubang Kerian, Malaysia.
Chan Yean YeanDepartment of Medical Microbiology and Parasitology, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, Kubang Kerian, Malaysia.
Nor Rosidah IbrahimDepartment of Pediatrics, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, Kubang Kerian, Malaysia.
Manal Abdel Haleem A AbusalahDepartment of Medical Microbiology and Parasitology, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, Kubang Kerian, Malaysia.
Zeehaida MohamedDepartment of Medical Microbiology and Parasitology, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, Kubang Kerian, Malaysia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Congenital and perinatal infections caused by pathogens such as Methods: The multiplex qPCR assay was optimized, and analytical performance, including sensitivity, specificity, and efficiency, was evaluated following Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines. Results: The assay demonstrated a limit of detection (LOD) of 10 attograms per microliter (ag/µL) for Conclusion: This multiplex qPCR assay provides highly sensitive and specific analytical performance for detecting key congenital pathogens. It represents a promising high-throughput tool with the potential to improve the turnaround time of neonatal molecular screening.

Indexed as

CytomegalovirusHerpesvirus 1, HumanHerpesvirus 2, HumanMultiplex Polymerase Chain ReactionReal-Time Polymerase Chain ReactionToxoplasmaTreponema pallidumCytomegalovirus InfectionsHumansInfant, NewbornSensitivity and SpecificityCMVCongenital infectionsHSVMolecular diagnosticsMultiplex qPCRNeonatal screeningToxoplasma gondiiTreponema pallidum

Identifiers

PMID42761597
PMCPMC13588099

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.