ArticleProceedings of the National Academy of Sciences of the United States of America2026
The structural basis of malodorant skatole formation by the glycyl radical enzyme indoleacetate decarboxylase.
Christa N Imrich, Lindsey R F Backman, Abigail P Allworth, Mary C Andorfer, Jared C Paris, Nina M Greeley, Beverly Fu, Emily P Balskus, Catherine L Drennan
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In one paragraphArticle in Proceedings of the National Academy of Sciences of the United States of America, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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5 · Who and what moneyAuthors and funding
9 authors.
Christa N ImrichDepartment of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139.
Lindsey R F BackmanDepartment of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139.ORCID 0000-0002-0323-1336 Abigail P AllworthMIT Summer Research Program in Biology, Massachusetts Institute of Technology, Cambridge, MA 02139.
Mary C AndorferDepartment of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139.
Jared C ParisDepartment of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139.
Nina M GreeleyDepartment of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139.
Beverly FuDepartment of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138.
Emily P BalskusDepartment of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138.ORCID 0000-0001-5985-5714 Catherine L DrennanDepartment of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139.ORCID 0000-0001-5486-2755 Funding
TRAINING GRANTS IN ENVIROMENTAL TOXICOLOGYT32ES007020 · NIEHS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI ESSIGMANN, JOHN M · 1985 to 2024
$13.1MMetalloenzyme structure, function and assemblyR35GM126982 · NIGMS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI CATHERINE L DRENNAN · 2018 to 2026
$3.4MInvestigation and application of hydrocarbon-degrading enzymes using cryo-electron microscopy and directed evolutionR00GM145910 · NIGMS · MICHIGAN STATE UNIVERSITY · PI ANDORFER, MARY CATHERINE · 2023 to 2025
$855kInvestigation and application of hydrocarbon-degrading enzymes using cryo-electron microscopy and directed evolutionK99GM145910 · NIGMS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI ANDORFER, MARY CATHERINE · 2022 to 2023
$112kHHMI (HHMI) Gates Faculty Scholar Award (OPP1158186)HHMI (HHMI) GilliamHHMI (HHMI) InvestigatorHHS | NIH (NIH) GM126982HHS | NIH (NIH) GM145910HHS | NIH (NIH) T32ES007020NIEHS NIH HHS T32 ES007020NIGMS NIH HHS K99 GM145910NIGMS NIH HHS R00 GM145910NIGMS NIH HHS R35 GM126982NSF (NSF) Waterman CHE-20380529NSF | NSF Graduate Research Fellowship Program (GRFP) 1122374NSF | NSF Graduate Research Fellowship Program (GRFP) 1144152
6 · The paper itselfAbstract
Glycyl radical enzymes (GREs) catalyze challenging chemical reactions using a posttranslationally installed glycyl radical cofactor. One such enzyme, indoleacetate decarboxylase (IAD), performs the radical-based decarboxylation of indole-3-acetate (I3A) to form the malodorant molecule skatole. In addition to being an odor nuisance, skatole is a human and livestock lung toxin, a suspected carcinogen, and a mosquito attractant, all of which impact human health, agriculture, food production, and wastewater treatment. Here, we use cryogenic electron microscopy to solve a 2.45-Å resolution structure of IAD from the gut bacterium
Indexed as
Bacterial ProteinsCarboxy-LyasesSkatoleCatalytic DomainCryoelectron MicroscopyDecarboxylationModels, MolecularBacterial ProteinsCarboxy-LyasesSkatoleanaerobic metabolismcryo-EMKolbe-type decarboxylationmicrobiomeradical chemistry
Identifiers
PMID42758728
PMCPMC13598205
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