Evidence map›Paper›PMID 42758586›Full record

ArticleSTAR protocols2026

Protocol for assessing protein expression levels and localization during fission yeast cytokinesis.

Jack R Gregory, Yanfang Ye, Davinder Singh, Aysha H Osmani, Jian-Qiu Wu

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Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Jack R GregoryDepartment of Molecular Genetics, The Ohio State University, Columbus, OH 43210, USA; Cellular, Molecular, and Biochemical Sciences Program, The Ohio State University, Columbus, OH 43210, USA; Graduate Program of Molecular, Cellular, and Developmental Biology, The Ohio State University, Columbus, OH 43210, USA. Electronic address: gregory.600@osu.edu.
Yanfang YeDepartment of Molecular Genetics, The Ohio State University, Columbus, OH 43210, USA.
Davinder SinghDepartment of Molecular Genetics, The Ohio State University, Columbus, OH 43210, USA; Postdoctoral Candidate Pelotonia Scholars Program, The Ohio State University, Columbus, OH 43210, USA.
Aysha H OsmaniDepartment of Molecular Genetics, The Ohio State University, Columbus, OH 43210, USA.
Jian-Qiu WuDepartment of Molecular Genetics, The Ohio State University, Columbus, OH 43210, USA. Electronic address: wu.620@osu.edu.

Funding

Molecular Mechanisms of CytokinesisR01GM118746 · NIGMS · OHIO STATE UNIVERSITY · PI WU, JIAN-QIU · 2016 to 2025
$2.7M
Cellular, molecular, and biochemical sciences training grantT32GM141955 · NIGMS · OHIO STATE UNIVERSITY · PI Jane Elizabeth Jackman, JESSE J KWIEK · 2021 to 2026
$2.2M
Molecular mechanisms of cytokinesis and plasma membrane repairR35GM164259 · NIGMS · OHIO STATE UNIVERSITY · PI Jian-Qiu Wu · 2026 to 2026
$417k
NIGMS NIH HHS R01 GM118746NIGMS NIH HHS R35 GM164259NIGMS NIH HHS T32 GM141955
6 · The paper itself

Abstract

The fission yeast Schizosaccharomyces pombe is a genetically tractable model that shares mechanisms for cytokinesis with higher eukaryotes. Here, we present a protocol for analyzing fission yeast cytokinesis proteins. We include steps for gene targeting by homologous recombination, functionality tests by genetic interactions, and imaging by high-spatiotemporal resolution microscopy. Next, we describe procedures for image analyses using Fiji, protein interactions by co-immunoprecipitation, and measuring protein levels by Western blotting. These techniques are transferable to other subcellular locations and model organisms. For complete details on the use and execution of this protocol, please refer to Ye et al.

Indexed as

Cell BiologyMicroscopyModel Organisms

Identifiers

PMID42758586
PMCPMC13602322

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.