Evidence map›Paper›PMID 42758585›Full record

ArticleSTAR protocols2026

Protocol for targeted analysis of oxylipins in rat plasma and mouse macrophage samples by high-resolution UHPLC-MS/MS.

Larissa R Diniz, Adriano B Chaves-Filho, Pedro H F Jordão, Rosangela S Santos, Marina T Botana, Johan Kolmert, Ana C A Zucão, Marteinn T Snaebjornsson, Eduardo M Reis, Almut Schulze and 3 more

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Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Larissa R DinizDepartamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, SP 05508-000, Brazil; Centro de Metabolismo (CoMETA), Hospital Universitário, Universidade de São Paulo, São Paulo, SP 05508-000, Brazil.
Adriano B Chaves-FilhoDivision of Tumor Metabolism and Microenvironment, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 581, 69120 Heidelberg, Germany.
Pedro H F JordãoDepartamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, SP 05508-000, Brazil.
Rosangela S SantosDepartamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, SP 05508-000, Brazil; Heidelberg University Biochemistry Center (BZH), Heidelberg University, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany.
Marina T BotanaUnit of Integrative Metabolomics, Institute of Environmental Medicine, Karolinska Institute, 17177 Stockholm, Sweden.
Johan KolmertUnit of Integrative Metabolomics, Institute of Environmental Medicine, Karolinska Institute, 17177 Stockholm, Sweden.
Ana C A ZucãoDepartamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, SP 05508-000, Brazil; Centro de Metabolismo (CoMETA), Hospital Universitário, Universidade de São Paulo, São Paulo, SP 05508-000, Brazil.
Marteinn T SnaebjornssonDivision of Tumor Metabolism and Microenvironment, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 581, 69120 Heidelberg, Germany.
Eduardo M ReisDepartamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, SP 05508-000, Brazil.
Almut SchulzeDivision of Tumor Metabolism and Microenvironment, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 581, 69120 Heidelberg, Germany.
Britta BrüggerHeidelberg University Biochemistry Center (BZH), Heidelberg University, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany.
Craig E WheelockUnit of Integrative Metabolomics, Institute of Environmental Medicine, Karolinska Institute, 17177 Stockholm, Sweden; Department of Respiratory Medicine and Allergy, Karolinska University Hospital, 17176 Stockholm, Sweden.
Sayuri MiyamotoDepartamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, SP 05508-000, Brazil; Centro de Metabolismo (CoMETA), Hospital Universitário, Universidade de São Paulo, São Paulo, SP 05508-000, Brazil. Electronic address: miyamoto@iq.usp.br.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Oxylipins are bioactive oxygenated fatty acid derivatives that act as signaling mediators in diverse physiological and pathological processes. Here, we present a protocol for the simultaneous analysis of 125 oxylipins in rat plasma and mouse macrophage samples. We describe steps for preparing standards and calibration curves, extracting oxylipins, and performing ultra-high-performance liquid chromatography coupled to high-resolution tandem mass spectrometry (UHPLC-MS/MS). We also detail procedures for using staRoxy, an original R package for oxylipin abundance data analysis. For complete details on the use and execution of this protocol, please refer to Chaves-Filho et al.,

Indexed as

Mass SpectrometryMetabolomicsProtocols in Metabolomics and Lipidomics

Identifiers

PMID42758585
PMCPMC13602319

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.