ArticleApplied biochemistry and biotechnology2026
Rapid Differential Detection of PRRSV NADC30-like and NADC34-like Strains Using a CRISPR-Cas13a/RT-RAA Assay.
Article in Applied biochemistry and biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The global swine industry suffers substantial economic losses from Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), which causes reproductive and respiratory disorders. With NADC30- and NADC34-like strains now predominant, differentiation is complicated by their similar biology, despite characteristic Nsp2 gene deletions of 131 aa and 100 aa, respectively, highlighting a need for improved diagnostics. Moving fundamentally beyond conventional broad-spectrum detection, this assay innovatively targets the characteristic Nsp2 deletion signatures. A diagnostic platform integrating CRISPR-Cas13a with recombinase-aided amplification (RT-RAA) was constructed. Utilising aligned Nsp2 sequences, conserved crRNAs, and RT-RAA primers, NADC30- and NADC34-like strains were evaluated for their assay time, specificity, sensitivity, and consistency with RT-qPCR. The assay achieved rapid, specific detection with no cross-reactivity to common swine pathogens, and highly sensitive detection limits of 1.7 × 10¹ and 2.3 × 10¹ copies/µL for NADC30- and NADC34-like strains, respectively. Clinical validation using 30 samples demonstrated complete concordance with RT-qPCR, and the integration of T7 transcription enabled the single-tube differentiation of both strains. In summary, this one-tube, two-step fluorescent system presents a robust alternative for PRRSV diagnostics, combining specificity, sensitivity, and ease of use. It is well-suited for rapid field identification, molecular typing, and surveillance, thereby aiding in the implementation of targeted control measures.
Indexed as
Identifiers
42758398What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.