ArticleISME communications2026
Overcoming gelling agent-derived inhibitory factors enables the cultivation of fastidious nitrifiers on solid media.
Article in ISME communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Abstract
Nitrification is a key process in the global nitrogen cycle, yet the physiological characterization of nitrifiers remains limited due to their low culturability. Although culture-independent approaches have provided important ecological insights, they do not fully resolve the physiological properties of nitrifying microorganisms. Here, we show that common gelling agents (agar, agarose, and gellan gum) contain toxic substances that inhibit the growth of diverse nitrifying microorganisms as well as some heterotrophic bacteria. These inhibitory substances appear to be generated during autoclaving and act via mechanisms distinct from previously reported hydrogen peroxide. Removal of these inhibitory factors using a simple washing strategy enables colony formation in recalcitrant nitrifiers, including
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