ArticleVeterinary and animal science2026
Methods for storage and determination of tryptophan and serotonin in bovine blood by high-performance liquid chromatography with fluorescence detection.
Article in Veterinary and animal science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Tryptophan (TRP) and serotonin (5-HT) play important biological roles, including neurotransmission. However, the preservation and quantification of TRP and 5-HT in blood samples are challenging due to their instability. In addition, methods for their determination in biological samples using high-performance liquid chromatography with fluorescence detection (HPLC-FLD) remain limited. This study aimed to develop methods for the preservation and quantification of 5-HT and TRP in blood using HPLC-FLD. Mobile phases with different pH values (3.5, 4.0, and 4.5) and methanol-to-buffer ratios (25:75, 20:80, and 15:85) were evaluated. A stability study using different preservatives (perchloric acid, oxalic acid, oxalic acid + glycine, EDTA, EDTA + perchloric acid, Na
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