ArticleFrontiers in microbiology2026
Functional characterization of GplA (MSMEG-0394) reveals a novel determinant of glycopeptidolipids homeostasis in
Article in Frontiers in microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Introduction: Glycopeptidolipids (GPLs) are major constituents of the cell envelope of non-tuberculous mycobacteria (NTM) and play essential roles in colony morphology, surface motility, biofilm formation, antimicrobial susceptibility, and host-pathogen interactions. Although the GPL biosynthetic locus has been extensively characterized, the function of several conserved genes within this cluster remains unresolved. Here, we functionally characterized GplA (MSMEG-0394), a conserved small protein encoded within the GPL biosynthetic locus of Methods: The role of GplA was investigated by combining genetic and phenotypic analyses, lipid profiling by thin-layer chromatography and electrospray ionization mass spectrometry, transcriptional analyses, complementation studies, and structural modelling. Results: Deletion of gplA resulted in extensive alterations of cell-envelope-associated phenotypes, including rough colony morphology, impaired sliding motility, delayed biofilm formation, increased cell aggregation, reduced growth, and enhanced susceptibility to rifampicin. Biochemical analyses demonstrated a marked remodeling of the GPL profile in the mutant strain, supporting a role for GplA in maintaining GPL homeostasis. Transcriptional analyses further showed that Discussion: Collectively, our findings identify GplA as a determinant of glycopeptidolipid homeostasis and suggest that this conserved small protein contributes to the coordination of cell-envelope composition, surface-associated physiology, and operon expression in
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.