ArticleSe pu = Chinese journal of chromatography2026
[Determination of two pancreatic stimulants in equine urine using ionic liquid-based deep eutectic solvent-assisted liquid-liquid microextraction and ultra-high performance liquid chromatography-tandem mass spectrometry].
Article in Se pu = Chinese journal of chromatography, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Acetohexamide （AH） and chlorpropamide （CP） can stimulate pancreatic secretion of insulin and regulate water-salt balance， which may indirectly lead to an increase in adrenaline and potentially enhance the athletic performance of horses. For the protection of horse welfare and the consideration of fair competition， AH and CP were listed as pancreatic stimulants by the Federation Equestre Internationale （FEI）. A method for the detection of pancreatic stimulants in equestrian sports needs to be established. Before analyzing these drugs from biological samples such as urine， it is necessary to perform pre-treatment to separate and concentrate the target analytes. However， there are several disadvantages to using traditional pre-treatment methods， including toxicity and inefficiency. A rapid and environmentally friendly pre-treatment method utilizing ionic liquid-based deep eutectic solvent vortex-assisted liquid-liquid microextraction （IL-DES-VALLME） was developed. Coupled with ultra-high performance liquid chromatography-tandem mass spectrometry （UHPLC-MS/MS）， the approach enabled efficient extraction and sensitive quantification of AH and CP from equine urine.
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