A Novel Synergistic Multilayer Nanofat Technique for Facial Rejuvenation.

backgroundNanofat is gaining traction in facial rejuvenation as a minimally invasive regenerative approach with short downtime and natural, sustained results. Despite growing evidence supporting its biological effects on multiple aspects of facial aging, its use remains limited. Non-surgical facial rejuvenation primarily relies on injectables, chemical peels, and energy-based procedures. However, most of these approaches address only selected aspects of aging and often require repeated treatments. This retrospective case series presents a novel synergistic multilayer nanofat protocol designed to address both skin quality and facial contour in a single minimally invasive session.

methodsA total of 42 consecutive patients treated between 2023 and 2025 were included. The protocol combined subcutaneous autologous nanofat injections with intradermal nanofat microneedling using an oscillating electronic device, with optional fractional CO

resultsModerate to marked overall improvement was observed in 35 of 42 patients (83%). Skin quality improved in all patients (42/42, 100%). Improved facial contour, particularly in the tear trough and midface, was noted in 38 of 42 patients (90%). Even low nanofat volumes were sufficient to achieve meaningful improvement in selected patients. Results remained stable at longer follow-up. No major complications occurred.

conclusionsThis retrospective analysis suggests that the novel synergistic multilayer nanofat approach may represent a feasible regenerative strategy for full-face rejuvenation. Further prospective studies are warranted. LEVEL OF EVIDENCE IV: This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266.