Evidence map›Paper›PMID 42752085›Full record

ArticleSTAR protocols2026

Protocol for collection and generation of bat primary lung and kidney cells.

Manuela M T Pereira, Rita M Quintela-Tizon, Amanda Vicente-Santos, Beckett L Olbrys, B R Ansil, Nancy B Simmons, Daniel J Becker, Kaushal Baid, Arinjay Banerjee

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Manuela M T PereiraVaccine and Infectious Disease Organization (VIDO), University of Saskatchewan, Saskatoon, SK S7N 5E3, Canada; Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada.
Rita M Quintela-TizonVaccine and Infectious Disease Organization (VIDO), University of Saskatchewan, Saskatoon, SK S7N 5E3, Canada; Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada.
Amanda Vicente-SantosSchool of Biological Sciences, University of Oklahoma, Norman, OK 73019, USA.
Beckett L OlbrysSchool of Biological Sciences, University of Oklahoma, Norman, OK 73019, USA.
B R AnsilSchool of Biological Sciences, University of Oklahoma, Norman, OK 73019, USA.
Nancy B SimmonsDepartment of Mammalogy, American Museum of Natural History, New York, NY 10024, USA.
Daniel J BeckerSchool of Biological Sciences, University of Oklahoma, Norman, OK 73019, USA. Electronic address: danbeck@ou.edu.
Kaushal BaidVaccine and Infectious Disease Organization (VIDO), University of Saskatchewan, Saskatoon, SK S7N 5E3, Canada. Electronic address: kaushal.baid@usask.ca.
Arinjay BanerjeeVaccine and Infectious Disease Organization (VIDO), University of Saskatchewan, Saskatoon, SK S7N 5E3, Canada; Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada; Department of Biology, University of Waterloo, Waterloo, ON N2L 3G1, Canada; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON M5S 1A8, Canada; Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC V6T 1Z, Canada. Electronic address: arinjay.banerjee@usask.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Some bat species host pathogenic viruses and represent recognized models for studying antiviral immunity. Here, we present a protocol for collecting and generating primary cells from wild bat tissues. We describe steps for tissue collection, enzymatic digestion, isolation, and cell expansion. We then detail procedures for immunocompetency testing of the established cultures. This protocol describes the generation of in vitro models for understudied non-model species, facilitating physiologically relevant assays to improve understanding of bat biology, immunology, and pathogen-host interactions.

Indexed as

Cell cultureCell isolationImmunologyMicrobiologyModel Organisms

Identifiers

PMID42752085
PMCPMC13601057

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.