Evidence map›Paper›PMID 42751795›Full record

ArticleActa crystallographica. Section F, Structural biology communications2026

Serendipitous crystal structure of mJuniper, a rationally designed cyan fluorescent protein.

Alpay Aydin, Nathan Fraikin, Priscillia Lagoutte, Christian Lesterlin, Laurent Terradot

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta crystallographica. Section F, Structural biology communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Alpay AydinMolecular Microbiology and Structural Biochemistry, UMR5086, Université de Lyon, CNRS, 69007 Lyon, France.
Nathan FraikinMolecular Microbiology and Structural Biochemistry, UMR5086, Université de Lyon, CNRS, 69007 Lyon, France.
Priscillia LagoutteMolecular Microbiology and Structural Biochemistry, UMR5086, Université de Lyon, CNRS, 69007 Lyon, France.
Christian LesterlinMolecular Microbiology and Structural Biochemistry, UMR5086, Université de Lyon, CNRS, 69007 Lyon, France.
Laurent TerradotMolecular Microbiology and Structural Biochemistry, UMR5086, Université de Lyon, CNRS, 69007 Lyon, France.ORCID 0000-0003-4279-9167

Funding

Ligue Contre le Cancer 2023
6 · The paper itself

Abstract

mJuniper is a recently developed monomeric cyan fluorescent protein (CFP) optimized for bacterial imaging, distinguished by the fastest maturation kinetics recorded for a CFP. mJuniper shows significant promise for time-lapse microscopy, but was used here to improve the solubility of a target protein. Here, we report the high-resolution X-ray structure of mJuniper, obtained serendipitously during an attempted structural characterization of CagG, an essential component of the Helicobacter pylori Cag type IV secretion system. The structure reveals a classical β-barrel scaffold, as well as a chromophore with two rotameric states, resulting from specific radiation damage occurring in its vicinity.

Indexed as

Bacterial ProteinsGreen Fluorescent ProteinsHelicobacter pyloriAmino Acid SequenceCrystallography, X-RayEscherichia coliModels, MolecularRecombinant ProteinsBacterial ProteinsCyan Fluorescent ProteinGreen Fluorescent ProteinsRecombinant Proteinschromophorefluorescent proteinsmJuniperserendipity

Identifiers

PMID42751795
PMCPMC13629639

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.