ArticleJournal of biomedical optics2026
Depth estimation from wavelength-dependent fluorescence spread profiles across NIR-I and NIR-II.
Article in Journal of biomedical optics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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20 authors.
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Abstract
Significance: Wide-field fluorescence-guided imaging is inherently depth ambiguous. Multispectral imaging across NIR-I and NIR-II may encode depth because longer-wavelength fluorescence undergoes less diffuse broadening in tissue. Aim: The study aims to determine whether wavelength-dependent radial spread profiles of subsurface indocyanine green (ICG) fluorescence can be used to estimate depth from a single excitation wavelength. Approach: ICG phantoms (1 to Results: Longer-wavelength channels produced more compact spread profiles, and full width at 20% peak intensity increased linearly with tissue thickness for all filters ( Conclusions: Wavelength-dependent fluorescence spread profiles across NIR-I and NIR-II can encode depth information for subsurface ICG targets, providing a wide-field, single-fluorophore, single-excitation approach compatible with emerging multispectral surgical imaging platforms.
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