Nonnative Thiol and Aniline Methylation, Including Enantioselective Dithiol Desymmetrization, by Multicomponent Cobamide-Dependent Methyltransferases Using Unactivated Methyl Donors.

Amardeep Kumar, Osman Ishag Adam, Paras Gupta, Jared C Lewis

Abstract read

In one paragraph

Article in Angewandte Chemie (International ed. in English), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

The Expanding Reactivity of Cobamide-Containing Proteins: From Mechanistic Understanding to Non-native Biocatalysis.ACS catalysis · 2026
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Amardeep KumarDepartment of Chemistry, Indiana University, Bloomington, Indiana, USA.ORCID https://orcid.org/0000-0002-5725-9262

Osman Ishag AdamDepartment of Chemistry, Indiana University, Bloomington, Indiana, USA.

Paras GuptaDepartment of Chemistry, Indiana University, Bloomington, Indiana, USA.

Jared C LewisDepartment of Chemistry, Indiana University, Bloomington, Indiana, USA.ORCID https://orcid.org/0000-0003-2800-8330

Funding

Engineering Halogenases for Small Molecule Functionalization and Enantioselective CatalysisR35GM152068 · NIGMS · TRUSTEES OF INDIANA UNIVERSITY · PI JARED C LEWIS · 2024 to 2026

$1.2M

NIGMS NIH HHS R35 GM152068NSF CHE-2154726US Army Research LaboratoryUS Army Research Office W911NF-22-1-0118US Army Research Office W911NF-26-1-A150

6 · The paper itself

Abstract

Despite their potential synthetic utility, cobamide-dependent MTases are relatively underutilized for biocatalysis. We developed mixed MtaABC/MtgABC enzyme platforms capable of catalyzing methylation of non-native thiol and aniline substrates using unactivated methyl donors like methanol and glycine betaine. This approach allows highly enantioselective and site-selective methylation of aliphatic thiols with high turnover number (up to 6000), providing the first demonstration of enantioselective non-native catalysis by cobamide-dependent enzymes. The MtaAC+MtgB system was subsequently engineered through site-directed mutagenesis, resulting in large enhancements in activity for the desymmetrization of non-native ester-containing prochiral thiols. These findings establish MTase complexes as a promising platform for non-native biocatalysis.

Indexed as

B12biocatalysiscobamidedesymmetrizationmethylation

Identifiers

PMID42750104

DOI10.1002/anie.1537547

PMCPMC13618148

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.