ArticleMolecular biotechnology2026
PCR-LbCas12a Assay for Genotyping of HPV 16 and 18.
Article in Molecular biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CRISPR/Cas12a systems have been widely used in nucleic acid detection, because of their high specificity and trans-cleavage activity. We combined the specificity of CRISPR/Cas12a system with the sensitivity of PCR, to develop a new HPV detection assay with high specificity and sensitivity, called PCR-LbCas12a assay. Here, we pre-amplified almost all high-risk HPV genotypes with consensus primer (MY09/11) and added to new tubes containing HPV 16 or 18 CRISPR/Cas12a system for HPV genotyping. The LOD of PCR-LbCas12a for HPV16 or HPV18 DNA was 100 copies and 10 copies per reaction, respectively. In mixed samples of HPV 16 and HPV 18 DNA, PCR-LbCas12a assay could detect HPV16 DNA from samples containing 10
Indexed as
Identifiers
42749982What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.