Evidence map›Paper›PMID 42747743›Full record

ArticleJournal of physiology and biochemistry2026

Zileuton protects pancreatic stellate cells from oxidative stress and ferroptosis by modulating the Nrf2 pathway and mitochondrial activity.

Matías Estarás, Cándido Ortiz-Placín, Salomé Martínez-Morcillo, Remigio Martínez, Miguel Fernández-Bermejo, Pablo Solís-Muñoz, José María Mateos, Juan Iovanna, Patricia Santofimia-Castaño, Antonio Gonzalez

Abstract read
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In one paragraph

Article in Journal of physiology and biochemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Matías EstarásUnidad de Gastroenterología, Complejo Hospitalario de Cáceres, Cáceres, Spain. matias.estaras@inserm.fr.
Cándido Ortiz-PlacínGrupo de Investigación Biología y Comunicación Celular. Departamento de Fisiología, Instituto de Biomarcadores de Patologías Moleculares, Facultad de Veterinaria, Universidad de Extremadura, Avenida de la Universidad s/n, Cáceres, Spain.
Salomé Martínez-MorcilloUnidad de Toxicología, Facultad de Veterinaria, Universidad de Extremadura, Avenida de la Universidad s/n, Cáceres, Spain.
Remigio MartínezDepartamento de Sanidad Animal, Facultad de Veterinaria, Universidad de Extremadura, Cáceres, Spain.
Miguel Fernández-BermejoUnidad de Gastroenterología, Complejo Hospitalario de Cáceres, Cáceres, Spain.
Pablo Solís-MuñozUnidad de Gastroenterología, Complejo Hospitalario de Cáceres, Cáceres, Spain.
José María MateosUnidad de Gastroenterología, Complejo Hospitalario de Cáceres, Cáceres, Spain.
Juan IovannaCentre de Recherche en Cancérologie de Marseille (CRCM), INSERM U1068, CNRS UMR 7258, Aix-Marseille Université and Institut Paoli- Calmettes, Parc Scientifique et Technologique de Luminy, 163 Avenue de Luminy, Marseille, 13288, France.
Patricia Santofimia-CastañoCentre de Recherche en Cancérologie de Marseille (CRCM), INSERM U1068, CNRS UMR 7258, Aix-Marseille Université and Institut Paoli- Calmettes, Parc Scientifique et Technologique de Luminy, 163 Avenue de Luminy, Marseille, 13288, France.
Antonio GonzalezGrupo de Investigación Biología y Comunicación Celular. Departamento de Fisiología, Instituto de Biomarcadores de Patologías Moleculares, Facultad de Veterinaria, Universidad de Extremadura, Avenida de la Universidad s/n, Cáceres, Spain. agmateos@unex.es.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Lipoxygenases exhibit a leading role in cancer development and growth. Additionally, pancreatic stellate cells (PSCs) are pivotal in the formation of fibrotic tissue in pancreatic tumors. In this work, we have studied the effects of the 5-lipoxygenase (5-LOX) inhibitor, zileuton, on rat PSCs. Cell viability was analyzed by employing crystal violet. Reactive oxygen species (ROS) generation, reduced (GSH) and oxidized (GSSG) levels of glutathione and protein and lipid oxidation were analyzed by fluorescence and colorimetric methods respectively. The phosphorylation of nuclear factor erythroid 2-related factor (Nrf2) and the expression of superoxide dismutase (SOD) was studied by western blotting. The effect of zileuton on erastin-induced ferroptosis was assessed by colorimetric methods. Mitochondrial metabolic activity was studied employing Alamar Blue

Indexed as

FerroptosisHydroxyureaLipoxygenase InhibitorsMitochondriaNF-E2-Related Factor 2Oxidative StressPancreatic Stellate CellsAnimalsAntioxidantsCell SurvivalGlutathioneMembrane Potential, MitochondrialPiperazinesRatsReactive Oxygen SpeciesSignal TransductionAntioxidantserastinGlutathioneHydroxyureaLipoxygenase InhibitorsNfe2l2 protein, ratNF-E2-Related Factor 2PiperazinesReactive Oxygen SpeciesSuperoxide DismutasezileutonAntioxidant enzymesCell viabilityFerroptosisGlutathioneNuclear factor erythroid 2-related factorPancreatic stellate cellsZileuton

Identifiers

PMID42747743
PMCPMC13582277

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.