ArticleTropical animal health and production2026
Association of polymorphisms in the ovine Leptin gene with body weight and body measurement traits in Balkhi sheep.
Article in Tropical animal health and production, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
The leptin gene was studied as a marker for productive traits in Balkhi sheep. A total of 60 blood samples were collected from Balkhi sheep, and body measurements and weights were recorded. In the CDS (coding sequence) region of the Leptin gene, a 471 bp fragment was amplified by PCR with a pair of (forward and reverse) specific primers. Two novel SNPs were detected at positions (g.348 G > T) and (g.394 G > A). The highest genotypic frequency at position g.348 G > T was found for genotype GG (71.67%), followed by GT (20.00%), and TT exhibited the lowest frequency (8.33%). A similar pattern was observed for SNP2 at g.394 G > A, with the same genotypic frequencies: GG (75%), GA (18.33%), and AA (6.67%). In SNP1, the frequency of the G allele was highest in the population, at 81.67%, and the T allele was 18.33%. Similarly, in SNP2, the G allele was 84.17%, and the A allele was 15.83%. Both SNPs were significantly associated with some economically important body measurement traits. The results of this study can be concluded that, among the three genotypes of SNP1 (g. 348G > T) and SNP2 (g. 394G > A), the TT and AA genotypes could be used for the traits of economic importance like body length, fat-tail thickness, fat-tail length, and withers height in Balkhi sheep through marker-assisted selection for future breeding programs.
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