Evidence map›Paper›PMID 42747600›Full record

ArticleTropical animal health and production2026

Effects of a multi-strain probiotic on production performance, antioxidant indices, intestinal morphology, and fecal microbiota in heat-stressed quails.

Mohammad Saeed Shahraki, Bahman Abdi-Hachesoo, Seyed Shahram Shekarforoush, Fatemeh Namazi

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Article in Tropical animal health and production, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

4 authors.

Mohammad Saeed ShahrakiAvian Diseases Research Center, Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, P.O. Box 1731, Shiraz, 71345, Iran.
Bahman Abdi-HachesooAvian Diseases Research Center, Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, P.O. Box 1731, Shiraz, 71345, Iran. abdi@shirazu.ac.ir.ORCID http://orcid.org/0000-0003-2997-9606
Seyed Shahram ShekarforoushDepartment of Food Hygiene and Public Health, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Fatemeh NamaziDepartment of pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.

Funding

School of Veterinary Science, Shiraz University 2GCB1M271378
6 · The paper itself

Abstract

This experiment aimed to assess the impact of a multi-strain probiotic cocktail containing spore-forming and lactic acid-producing species on the growth performance, intestinal morphometry, antioxidant indices, and fecal bacterial populations of Japanese quails reared under heat stress (HS) conditions. A total of 400 one-day-old unsexed Japanese quails were randomly allocated to four experimental groups (100 birds per group; four replicates of 25 birds each): (1) Control (normal temperature), (2) Probiotic (normal temperature + probiotic), (3) Heat stress (HS), and (4) HS + Probiotic. The probiotic cocktail, containing six bacterial strains, was administered via drinking water at a concentration of 1 g/L (equivalent to 6 × 10⁹ CFU/g) from day 1 to day 42. Heat stress was applied from day 21 to day 42 by exposing birds to 38 ± 2 °C for 8 h daily, while the control groups were maintained at 24 ± 2 °C. The HS+Probiotic group exhibited significantly lower final body weight compared to the other groups (P < 0.05), and the lowest feed intake and highest feed conversion ratio (FCR) were observed in the HS and HS+Probiotic groups, respectively. The HS and HS+probiotic groups had significantly lower serum total antioxidant capacity (TAC) levels compared to the probiotic group (P < 0.05). The highest length of villus in both the jejunum and ileum was observed in the probiotic-treated groups (P < 0.05). In contrast, the villus length-to-crypt depth (VL/CD) ratio in the jejunum was significantly lower in the HS group than in the other experimental groups (P < 0.05). Fecal total bacteria and fecal Enterobacteriaceae counts were notably lower in the probiotic group (P < 0.05). The HS group showed a notably lower spore-forming bacterial count than the control (P < 0.05). It is concluded that adding probiotics to the drinking water of heat-stressed quails improved intestinal morphometry but did not significantly enhance production performance.

Indexed as

AntioxidantsCoturnixGastrointestinal MicrobiomeIntestinesProbioticsAnimalsFecesHeat-Shock ResponseHot TemperatureMaleRandom AllocationAntioxidantsEnterobacteriaceaeGut integrityHeat stressJapanese quailMulti-strain probioticOxidative status

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.