Evidence map›Paper›PMID 42747590›Full record

ArticleActa parasitologica2026

Integrating Community Practices, Vector Resistance, and Molecular Xenomonitoring of Wuchereria Bancrofti: A One Health Implementation Study in Lymphatic Filariasis-Endemic Communities.

Musa Abubakar Yalwa, Mukhtar Aliyu, Aminu Isa Musa, Ibrahim Malami, Murtala Bello Abubakar, Iliya Shehu Ndams, Aliyu Muhammad

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Article in Acta parasitologica, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Musa Abubakar YalwaDepartment of Biochemistry, Ahmadu Bello University, Zaria, Kaduna State, Nigeria.
Mukhtar AliyuDepartment of Biochemistry, Ahmadu Bello University, Zaria, Kaduna State, Nigeria.
Aminu Isa MusaDepartment of Biochemistry, Ahmadu Bello University, Zaria, Kaduna State, Nigeria.
Ibrahim MalamiCentre for Advanced Medical Research & Training (CAMRET), Usmanu Danfodiyo University, Sokoto, Nigeria.
Murtala Bello AbubakarDepartment of Physiology, College of Medicine and Health Sciences, Sultan Qaboos University, Muscat, Oman.
Iliya Shehu NdamsAfrica Centre of Excellence for Neglected Tropical Diseases and Forensic Biotechnology, Ahmadu Bello University, Zaria, Nigeria.
Aliyu MuhammadDepartment of Biochemistry, Ahmadu Bello University, Zaria, Kaduna State, Nigeria. amachida31@gmail.com.ORCID https://orcid.org/0000-0002-4167-7793

Funding

Africa Center of Excellence for Neglected Tropical Diseases and Forensic Biotechnology P21LSBC8022
6 · The paper itself

Abstract

purposeLymphatic filariasis (LF), caused primarily by Wuchereria bancrofti and transmitted by mosquitoes, remains a public health challenge in Nigeria. Insecticides and insecticide-treated nets (ITNs) are used as control measures. However, limited public awareness and widespread insecticide resistance impede elimination. Molecular xenomonitoring is essential for detecting active transmission and determining when it is halted. This study assessed community knowledge, attitudes, and practices (KAPs) on LF, insecticide resistance, and xenomonitoring of W. bancrofti in Bodinga Local Government Area (LGA).

methodsA semi-structured questionnaire was administered to 258 participants from three wards to assess KAPs. Anopheles and Culex larvae were collected, reared, and tested for susceptibility to deltamethrin, permethrin, and alphacypermethrin using the Centre for Disease Control (CDC) bioassay. Comparative expression of cytochrome P450 genes (CYP6M2, CYP9M10) was also evaluated. Blood-fed mosquitoes were collected with a Prokopack aspirator for microfilaria screening and molecular xenomonitoring using polymerase chain reactions (PCRs).

resultsOnly 41.9% of respondents had heard of LF, indicating significant knowledge gaps. Anopheles and Culex were resistant to all pyrethroids (6-44% mortality). Piperonyl butoxide (PBO) restored susceptibility to alphacypermethrin (98-99%). Of 1,510 mosquitoes, 5 (0.33%) were detected as infected by microscopy; 3 (0.20%) were PCR-confirmed W. bancrofti, and the L3 larvae was detected using quantitative reverse transcription PCR (qRT-PCR). Real-time PCR of microscopy-negative pools showed no infections. CYP6M2 and CYP9M10 were downregulated (0.26-fold and 0.77-fold, respectively) in infected mosquitoes.

conclusionThe study highlights low LF knowledge, pyrethroid resistance, and possible active transmission in Bodinga LGA. Awareness campaigns, PBO-based interventions, and further resistance mechanism studies are recommended.

Indexed as

AnophelesElephantiasis, FilarialInsecticide ResistanceMosquito VectorsWuchereria bancroftiAnimalsCulexCytochrome P-450 Enzyme SystemFemaleHealth Knowledge, Attitudes, PracticeHumansInsecticidesMaleMiddle AgedNigeriaPyrethrinsCytochrome P-450 Enzyme SystemInsecticidesPyrethrinsCytochrome P450sInsecticide resistanceLymphatic filariasis (LF)Molecular xenomonitoringWuchereria bancrofti

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PMID42747590

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