Evidence map›Paper›PMID 42745954›Full record

ArticleNAR genomics and bioinformatics2026

Customizable host and viral transcript enrichment using CRISPR-Cas9 long-read sequencing for characterization of low-to-moderate abundance isoforms.

An N T Nguyen, Jianshu Zhang, Shuxin Zhang, Miranda E Pitt, Devika Ganesamoorthy, Svenja Fritzlar, Jessie J-Y Chang, Sarah L Londrigan, Lachlan J M Coin

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In one paragraph

Article in NAR genomics and bioinformatics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

An N T NguyenDepartment of Microbiology and Immunology, The University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Victoria 3000, Australia.
Jianshu ZhangDepartment of Microbiology and Immunology, The University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Victoria 3000, Australia.
Shuxin ZhangDepartment of Microbiology and Immunology, The University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Victoria 3000, Australia.
Miranda E PittDepartment of Microbiology and Immunology, The University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Victoria 3000, Australia.
Devika GanesamoorthyChildren's Intensive Care Research Program, Child Health Research Centre, The University of Queensland, Brisbane 4101, Australia.
Svenja FritzlarDepartment of Microbiology and Immunology, The University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Victoria 3000, Australia.
Jessie J-Y ChangDepartment of Microbiology and Immunology, The University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Victoria 3000, Australia.
Sarah L LondriganDepartment of Microbiology and Immunology, The University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Victoria 3000, Australia.
Lachlan J M CoinDepartment of Microbiology and Immunology, The University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Victoria 3000, Australia.ORCID https://orcid.org/0000-0002-4300-455X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

One of the main challenges of whole transcriptome sequencing is the difficulty in detecting and quantifying low-to-moderate abundance transcripts. Methods that address this are either complicated to scale or customize; long-range PCR is problematic to scale, and probe hybridization panels are expensive to customize. In this study, we developed an RNA-guided CRISPR-Cas9 nuclease-based enrichment strategy combined with long-read sequencing, which achieved up to 60-fold enrichment of the target. Our findings demonstrate that the CRISPR-Cas system is a highly effective method for customizable long-read sequencing of target transcripts, which preserves estimation of relative abundance.

Indexed as

CRISPR-Cas SystemsSequence Analysis, RNAHigh-Throughput Nucleotide SequencingHumansProtein IsoformsProtein Isoforms

Identifiers

PMID42745954
PMCPMC13575421

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