Evidence map›Paper›PMID 42745639›Full record

ArticleBiotechnology journal2026

Enhancing the Scalable Manufacturing of Human Mesenchymal Stromal Cells in Dynamic Culture Using Bioactive Heparin/Collagen Layer-by-Layer Coated Microcarriers.

Justin Putman, Varsha Karunakaran, Roaa Hadi, Luis Pinzon-Herrera, Thallapuranam Krishnaswamy Suresh Kumar, Younghye Song, Jorge Almodovar

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biotechnology journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Justin PutmanDepartment of Biomedical Engineering, College of Engineering, University of Arkansas, Fayetteville, Arkansas, USA.
Varsha KarunakaranDepartment of Chemistry & Biochemistry, Fulbright College of Arts & Sciences, University of Arkansas, Fayetteville, Arkansas, USA.
Roaa HadiCell and Molecular Biology Graduate Program, Department of Biomedical Engineering, College of Engineering, University of Arkansas, Fayetteville, Arkansas, USA.
Luis Pinzon-HerreraDepartment of Chemical, Biochemical, and Environmental Engineering, College of Engineering and Information Technology, University of Maryland Baltimore County, Baltimore, Maryland, USA.
Thallapuranam Krishnaswamy Suresh KumarDepartment of Chemistry & Biochemistry, Fulbright College of Arts & Sciences, University of Arkansas, Fayetteville, Arkansas, USA.ORCID https://orcid.org/0000-0002-7574-3226
Younghye SongDepartment of Biomedical Engineering, College of Engineering, University of Arkansas, Fayetteville, Arkansas, USA.ORCID https://orcid.org/0000-0002-8391-7556
Jorge AlmodovarDepartment of Chemical, Biochemical, and Environmental Engineering, College of Engineering and Information Technology, University of Maryland Baltimore County, Baltimore, Maryland, USA.ORCID https://orcid.org/0000-0002-1151-3878

Funding

Unraveling Gene-Environment Interactions Shaping Metabolism: A Multi-Omics Analysis in DrosophilaP20GM139768 · NIGMS · UNIVERSITY OF ARKANSAS AT FAYETTEVILLE · PI Joanna Fiddler · 2021 to 2026
$17.0M
National Science Foundation 2051582NIGMS NIH HHS P20 GM139768NIH HHS P20GM139768
6 · The paper itself

Abstract

Expansion of human mesenchymal stromal cells (hMSCs) remains a bottleneck during manufacturing, where high cell yields must be achieved without compromising quality. Microcarriers provide elevated surface areas but often rely on synthetic materials. Here we use heparin/collagen Layer-by-Layer (LbL) coatings on microcarriers to enhance dynamic hMSC culture. HMSCs were cultured on LbL-coated and untreated microcarriers in up to 500 mL flasks, and were assessed over time, up to 18 days across three seeding densities (2600-20,000 cells/cm

Indexed as

Cell Culture TechniquesCollagenHeparinMesenchymal Stem CellsCell ProliferationCells, CulturedCoated Materials, BiocompatibleHumansCoated Materials, BiocompatibleCollagenHeparincollagendynamic cultureheparinhMSCslayer‐by‐layer coatingmicrocarriers

Identifiers

PMID42745639
PMCPMC13579213

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.